Abraham S, Rull J, Mendoza M, Liendo M C, Devescovi F, Roriz A K, Kovaleski A, Segura D F, Vera M T
Laboratorio de Investigaciones Ecoetológicas de Moscas de la Fruta y sus Enemigos Naturales (LIEMEN), PROIMI, Tucumán, Argentina.
Instituto de Ecología, A.C., Xalapa, Veracruz, Mexico.
Bull Entomol Res. 2014 Jun;104(3):376-82. doi: 10.1017/S0007485314000200. Epub 2014 Apr 4.
The South American fruit fly, Anastrepha fraterculus, is a complex of cryptic species composed of at least seven morphotypes. Some of them, such as the Peruvian and Brazilian 1 morphotypes (which include Argentinean populations), exhibit strong pre-copulatory isolation, yet it is possible to obtain heterotypic crosses when forcing copulation of adults under laboratory conditions. The cross involving Peruvian males and Argentinean females produces F1 offspring with reduced viability in terms of egg hatch. This low hatchability could be caused by a reduced amount of sperm transferred to and stored by females mated with heterotypic males, which in turn could affect their post-copulatory behaviour. To test these hypotheses, we investigated sperm transfer and female mating and remating behaviour for homotypic and heterotypic crosses between adults of two morphotypes (Brazilian 1 [Argentina] and Peruvian [Peru]) of the A. fraterculus cryptic species complex. As reported before, Argentinean males and females mated earlier in the day than the other three mating combinations. Peruvian females engaged in shorter copulation times than Argentinean females. Peruvian females tended to store smaller quantities of sperm than Argentinean females, and almost a half of the crosses involving Argentinean males and Peruvian females were unsuccessful (no sperm transfer). However, there was no evidence that the cross between Peruvian males and Argentinean females resulted in storage of a critically small amount of sperm (posing risk of sperm shortage). Argentinean females were more willing to remate than Peruvian females, irrespective of male morphotype, but latency to remating was not affected by male or female morphotype. This study shows that mating behaviour differs between some of the A. fraterculus complex morphotypes, with female but not male morphotype determining female likelihood to remate.
南美果蝇（Anastrepha fraterculus）是一个由至少七种形态型组成的隐存种复合体。其中一些，如秘鲁和巴西1形态型（包括阿根廷种群），表现出强烈的交配前隔离，但在实验室条件下强迫成虫交配时，仍有可能获得异型杂交后代。涉及秘鲁雄蝇和阿根廷雌蝇的杂交产生的F1代后代在卵孵化方面的活力降低。这种低孵化率可能是由于与异型雄蝇交配的雌蝇转移和储存的精子数量减少所致，这反过来可能会影响它们的交配后行为。为了验证这些假设，我们研究了果蝇隐存种复合体的两种形态型（巴西1[阿根廷]和秘鲁[秘鲁]）成虫之间同型和异型杂交中的精子转移以及雌蝇的交配和再交配行为。如之前报道的那样，阿根廷的雄蝇和雌蝇比其他三种交配组合更早开始交配。秘鲁雌蝇的交配时间比阿根廷雌蝇短。秘鲁雌蝇储存的精子数量往往比阿根廷雌蝇少，并且几乎一半涉及阿根廷雄蝇和秘鲁雌蝇的杂交未成功（没有精子转移）。然而，没有证据表明秘鲁雄蝇和阿根廷雌蝇之间的杂交导致储存的精子数量极少（存在精子短缺风险）。无论雄蝇形态型如何，阿根廷雌蝇比秘鲁雌蝇更愿意再交配，但再交配潜伏期不受雄蝇或雌蝇形态型的影响。这项研究表明，果蝇复合体中某些形态型之间交配行为存在差异，雌蝇而非雄蝇的形态型决定了雌蝇再交配的可能性。
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