具有多组氨酸结构域的弹性蛋白样多肽生物聚合物与银的杀菌活性

Bactericidal activity of elastin-like polypeptide biopolymer with polyhistidine domain and silver.

作者信息

Krishnani Kishore K, Chen Wilfred, Mulchandani Ashok

机构信息

Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.

Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA; Department of Chemical and Biomolecular Engineering, University of Delaware, DE, USA.

出版信息

Colloids Surf B Biointerfaces. 2014 Jul 1;119:66-70. doi: 10.1016/j.colsurfb.2014.03.018. Epub 2014 Mar 16.

DOI:10.1016/j.colsurfb.2014.03.018

PMID:24703635

Abstract

In the present study, elastin-like biopolymer (ELP) composed of a polyhistidine domain has been investigated as a silver binding agent for antibacterial activity against Escherichia coli, a model test strain for Gram-negative bacteria for antibacterial assays of nanoparticles, and Vibrio harveyi, an opportunistic pathogen which cause mass mortality in shrimp Penaeus monodon reared in coastal aquaculture. The concentration dependent antimicrobial activity of ELPH-Ag on E. coli and V. harveyi was examined by agar well diffusion method and further confirmed through growth curves using spectrophotometer assisted absorption observations. The increased concentrations of ELP-Ag effectively checked the bacterial growth and increased the diameter of inhibition zone. The results showed a minimum inhibitory concentration of 37 μg/ml. This study has an application in formulating artificial protein based antibacterial in diverse fields of healthcare and management of disease in coastal aquaculture.

摘要

在本研究中，由多组氨酸结构域组成的类弹性蛋白生物聚合物（ELP）已被作为一种银结合剂进行研究，用于对大肠杆菌（纳米颗粒抗菌测定中革兰氏阴性菌的模型测试菌株）和哈维氏弧菌（在沿海水产养殖中养殖的斑节对虾中导致大量死亡的机会致病菌）的抗菌活性。通过琼脂孔扩散法检测了ELPH-Ag对大肠杆菌和哈维氏弧菌的浓度依赖性抗菌活性，并使用分光光度计辅助吸收观察通过生长曲线进一步证实。ELP-Ag浓度的增加有效地抑制了细菌生长并增加了抑菌圈直径。结果显示最低抑菌浓度为37μg/ml。本研究在医疗保健的各个领域以及沿海水产养殖疾病管理中基于人工蛋白质的抗菌制剂配方方面具有应用价值。