Cung M T, Marraud M, Hadjidakis I, Bairaktari E, Sakarellos C, Kokla A, Tzartos S
Biopolymers. 1989 Jan;28(1):465-78. doi: 10.1002/bip.360280141.
A comparative 1H-NMR spectral study of a synthetic decapeptide containing the main immunogenic region of the Torpedo acetylcholine receptor (AChR; WNPADYGGIK, representing the alpha 67-76 fragment of Torpedo AChR) with four analogous peptides (WNP3-D5YGGIK, WNPAA5YGGIK, WNPADYGGA9K, and WNPD4DYGGV9K) has been carried out in dimethyl sulfoxide. One- and two-dimensional nmr experiments [correlated spectroscopy (COSY), relayed COSY, and phase-sensitive nuclear Overhauser enhancement spectroscopy (NOESY)] were performed to obtain complete assignments of the proton resonances. The presence of strong and multiple short- and long-range NOEs, and especially a strong long-range NOE between the two Asn2-C alpha H and Gly7-C alpha H protons, argues in favor of a rigid folded structure in all five cases. Temperature dependence measurements indicate the existence of three intramolecular interactions involving the Asp3, Gly8, and Lys10 amide protons.
在二甲基亚砜中对一种包含电鳐乙酰胆碱受体（AChR；WNPADYGGIK，代表电鳐AChR的α67 - 76片段）主要免疫原性区域的合成十肽与四种类似肽（WNP3 - D5YGGIK、WNPAA5YGGIK、WNPADYGGA9K和WNPD4DYGGV9K）进行了比较1H - NMR光谱研究。进行了一维和二维核磁共振实验[相关光谱（COSY）、中继COSY和相敏核Overhauser增强光谱（NOESY）]以获得质子共振的完整归属。在所有五种情况下，存在强烈且多个短程和长程核Overhauser效应（NOE），特别是两个Asn2 - CαH和Gly7 - CαH质子之间强烈的长程NOE，这表明存在刚性折叠结构。温度依赖性测量表明存在涉及Asp3、Gly8和Lys10酰胺质子的三种分子内相互作用。
