MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Biosens Bioelectron. 2014 Aug 15;58:388-94. doi: 10.1016/j.bios.2014.02.082. Epub 2014 Mar 12.
MicroRNAs play pivotal roles in many fundamental aspects of life. Because microRNAs have the characteristics of small size, similar sequence, and low abundance, it is challenging to identify microRNAs rapidly and specifically with high sensitivity. Herein, we developed an electrochemiluminescent (ECL) chip system for microRNA detection based on base-stacking hybridization and magnetic microparticle enrichment technology. In the designed system, the integration of the microfluidic system with ECL detection made it easy to assemble the multiple assay steps and allowed the construction of a device that is convenient to carry. A limit of detection of 1fmol was achieved with this assay. The proposed direct optical microRNA detection technique demonstrated an acceptable sensitivity combined with the advantages of reliability and rapidity.
微小 RNA 在生命的许多基本方面发挥着关键作用。由于微小 RNA 具有体积小、序列相似和丰度低的特点，因此快速、特异性和高灵敏度地识别微小 RNA 具有挑战性。在此，我们开发了一种基于碱基堆积杂交和磁性微粒富集技术的用于微小 RNA 检测的电化学发光 (ECL) 芯片系统。在设计的系统中，微流控系统与 ECL 检测的集成使得多个检测步骤的组装变得容易，并允许构建便于携带的设备。该检测方法的检测限达到 1fmol。所提出的直接光学微小 RNA 检测技术结合了可靠性和快速性的优点，具有可接受的灵敏度。
