University College London, Department of Biochemical Engineering, London, United Kingdom.
Biotechnol J. 2014 Jun;9(6):717-8. doi: 10.1002/biot.201400098. Epub 2014 Apr 7.
Recent advances in cell culture have reduced upstream costs, and made downstream processing the more expensive component of biologics manufacturing. Within downstream processing, the chromatographic steps have usually been the most expensive, on the one hand because chromatographic steps are traditionally run in batch mode and on the other because resins are typically more expensive per unit mass of product than other bioproduct-contact materials [1, 2]. There have been many attempts to consider alternatives to chromatography [3, 4], but the comparatively high resolution of chromatography, combined with the bioprocess industry's wealth of experience in using the various chromatographic modes, have made it hard to replace in a manufacturing setting. In this issue of Biotechnology Journal Hammerschmid et al. [5] make several original contributions to this discussion.
近年来细胞培养的进步降低了上游成本，使下游处理成为生物制品制造中更昂贵的环节。在下游处理中，色谱步骤通常是最昂贵的，一方面是因为色谱步骤传统上以批处理模式运行，另一方面是因为树脂每单位产品质量比其他生物制品接触材料通常更昂贵[1,2]。已经有很多尝试考虑替代色谱法的方法[3,4]，但色谱法相对较高的分辨率，加上生物工艺行业在使用各种色谱模式方面的丰富经验，使得它在制造环境中难以被取代。在本期《生物技术杂志》上，Hammerschmid 等人[5]对这一讨论做出了几项原创贡献。
