Gim Sang-A, Koh Phil-Ok
Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, Korea.
Lab Anim Res. 2014 Mar;30(1):8-13. doi: 10.5625/lar.2014.30.1.8. Epub 2014 Mar 24.
Ferulic acid is known to act as a protective agent in cerebral ischemia through its anti-oxidant activity. γ-Enolase is a neuron-specific enolase that also exerts a neuroprotective effect. Here, we investigated whether ferulic acid regulates the expression level of γ-enolase in middle cerebral artery occlusion (MCAO)-induced brain injury and glutamate exposure-induced neuronal cell death. Adult male rats were treated with either vehicle or ferulic acid (100 mg/kg, i.v.) after MCAO and cerebral cortex tissues were collected 24 h after MCAO. Using a proteomics approach, we found that γ-enolase expression was decreased in MCAO-injured animals treated with vehicle alone, whereas ferulic acid treatment attenuated this decrease. Reverse-transcription PCR and Western blot analyses confirmed that ferulic acid treatment prevented MCAO injury-induced decrease in γ-enolase. Furthermore, in hippocampal-derived cell lines, glutamate exposure also decreased γ-enolase expression and ferulic acid treatment attenuated this glutamate-induced decrease in γ-enolase. These findings suggest that ferulic acid mediates a neuroprotective effect by attenuating injury-induced decreases of γ-enolase expression in neuronal cells.
阿魏酸因其抗氧化活性而被认为是脑缺血中的一种保护剂。γ-烯醇化酶是一种神经元特异性烯醇化酶，也具有神经保护作用。在此，我们研究了阿魏酸是否能调节大脑中动脉闭塞（MCAO）诱导的脑损伤和谷氨酸暴露诱导的神经元细胞死亡中γ-烯醇化酶的表达水平。成年雄性大鼠在MCAO后接受溶剂或阿魏酸（100mg/kg，静脉注射）处理，并在MCAO后24小时收集大脑皮质组织。使用蛋白质组学方法，我们发现单独用溶剂处理的MCAO损伤动物中γ-烯醇化酶表达降低，而阿魏酸处理减弱了这种降低。逆转录PCR和蛋白质印迹分析证实，阿魏酸处理可防止MCAO损伤诱导的γ-烯醇化酶降低。此外，在海马来源的细胞系中，谷氨酸暴露也降低了γ-烯醇化酶的表达，而阿魏酸处理减弱了谷氨酸诱导的γ-烯醇化酶降低。这些发现表明，阿魏酸通过减弱神经元细胞中损伤诱导的γ-烯醇化酶表达降低来介导神经保护作用。
