从荆香薷（Mosla chinensis Maxim. cv. Jiangxiangru）中提取的果胶多糖的结构和生物活性。
Structure and biological activities of a pectic polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph N1G 5C9, Ontario, Canada.
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph N1G 5C9, Ontario, Canada.
出版信息
Carbohydr Polym. 2014 May 25;105:276-84. doi: 10.1016/j.carbpol.2014.01.081. Epub 2014 Jan 31.
A water-soluble pectic polysaccharide (MP-A40) was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru for the first time, with a molecular weight of 32,600Da. MP-A40 was comprised of 68.63% galacturonic acid and 13.05% neutral sugar. In addition, arabinose, galactose, rhamnose, mannose and glucose composed the neutral sugar in a relative ratio of 4.94, 3.07, 2.13, 1.62 and 1.29% of the dry weight of MP-A40, respectively. Structural characterization of MP-A40 was investigated by methylation analysis and 1D/2D NMR spectroscopy. From the results, the structure of MP-A40 was revealed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare t-Araf (0.60%), t-Rhap (1.67%) and t-GalpA (10.15%). Esterification assay showed that about 32% of the carboxylic groups in GalA residues existed as methyl ester. In addition, MP-A40 could inhibit the growth of human leukemic cell line K562 and stimulate nitric oxide production from RAW 264.7 macrophages both in dose-dependent manners.
首次从荆香薷中分离得到一种水溶性的果胶多糖（MP-A40），其分子量为 32600Da。MP-A40 由 68.63%半乳糖醛酸和 13.05%中性糖组成。此外，阿拉伯糖、半乳糖、鼠李糖、甘露糖和葡萄糖以相对于 MP-A40 干重 4.94%、3.07%、2.13%、1.62%和 1.29%的比例构成中性糖。通过甲基化分析和 1D/2D NMR 光谱对 MP-A40 的结构进行了表征。结果表明，MP-A40 的结构如下：1,4-连接的α-d-GalpA 和 1,4-连接的α-d-GalpA6Me 与罕见的 t-Araf（0.60%）、t-Rhap（1.67%）和 t-GalpA（10.15%）交错。酯化试验表明，GalA 残基中约 32%的羧酸基以甲酯形式存在。此外，MP-A40 可以以剂量依赖的方式抑制人白血病细胞系 K562 的生长并刺激 RAW 264.7 巨噬细胞产生一氧化氮。
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