Fluer F S, Pozhar P F, Ratgauz G L, Akatov A K
Lab Delo. 1989(4):62-4.
The authors suggest a simple and rapid technique, staphylococci coagglutination, for rapid detection of the toxic shock exotoxin (TSE)-producing S. aureus strains. The method is based on the sensitization of formalin-treated Cowan-1 S. aureus cells with immunoglobulins to TSE. The reagent thus obtained coagglutinates with the supernatant of TSE-producing S. aureus tested strains. This method is more sensitive than the routinely used gel immunoprecipitation. The technique for preparing the supernatant of S. aureus tested cultures is also described.
作者们提出了一种简单快速的技术——葡萄球菌凝聚反应，用于快速检测产生中毒性休克外毒素（TSE）的金黄色葡萄球菌菌株。该方法基于用针对TSE的免疫球蛋白致敏经福尔马林处理的考恩1型金黄色葡萄球菌细胞。由此获得的试剂与产生TSE的金黄色葡萄球菌测试菌株的上清液发生凝聚反应。该方法比常规使用的凝胶免疫沉淀法更灵敏。文中还描述了制备金黄色葡萄球菌测试培养物上清液的技术。
