Department of Biology, University of Florida, Gainesville, Florida 32611, USA.
School of Biological and Chemical Sciences, Queen Mary, University of London, UK.
Genes (Basel). 2010 Jul 2;1(2):166-92. doi: 10.3390/genes1020166.
The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation (FISH) and genomic in situ hybridisation (GISH) have been applied to: 1) studies of interspecific hybridisation and polyploidy in nature, 2) analyses of phylogenetic relationships between species, 3) genetic mapping and 4) analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing (NGS) technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants.
不同谱系在种间杂交时的趋同，包括在多倍体（异源多倍体）增加时的趋同，是许多植物物种起源的驱动力。在植物育种中，种间杂交和异源多倍体同样重要，因为它们促进了外来 DNA 向育种系的渗入，从而引入新的特性。在这里，我们回顾了荧光原位杂交（FISH）和基因组原位杂交（GISH）如何应用于：1）自然种间杂交和多倍体的研究，2）物种间系统发育关系的分析，3）遗传作图，以及 4）植物育种材料的分析。我们还回顾了 FISH 如何利用下一代测序（NGS）技术，帮助快速表征自然种群和农业植物基因组中的重复部分。
