Rasala Beth A, Chao Syh-Shiuan, Pier Matthew, Barrera Daniel J, Mayfield Stephen P
California Center for Algae Biotechnology and Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America.
PLoS One. 2014 Apr 7;9(4):e94028. doi: 10.1371/journal.pone.0094028. eCollection 2014.
Transgenic microalgae have the potential to impact many diverse biotechnological industries including energy, human and animal nutrition, pharmaceuticals, health and beauty, and specialty chemicals. However, major obstacles to sophisticated genetic and metabolic engineering in algae have been the lack of well-characterized transformation vectors to direct engineered gene products to specific subcellular locations, and the inability to robustly express multiple nuclear-encoded transgenes within a single cell. Here we validate a set of genetic tools that enable protein targeting to distinct subcellular locations, and present two complementary methods for multigene engineering in the eukaryotic green microalga Chlamydomonas reinhardtii. The tools described here will enable advanced metabolic and genetic engineering to promote microalgae biotechnology and product commercialization.
转基因微藻有可能影响许多不同的生物技术产业，包括能源、人类和动物营养、制药、健康与美容以及特种化学品。然而，藻类复杂的基因和代谢工程面临的主要障碍是缺乏特征明确的转化载体来将工程基因产物导向特定的亚细胞位置，以及无法在单个细胞中强有力地表达多个核编码转基因。在此，我们验证了一组能使蛋白质靶向不同亚细胞位置的遗传工具，并提出了两种用于真核绿藻莱茵衣藻多基因工程的互补方法。本文所述的工具将推动先进的代谢和基因工程，以促进微藻生物技术和产品商业化。
