Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
Curr Opin Biotechnol. 2013 Apr;24(2):239-46. doi: 10.1016/j.copbio.2012.11.006. Epub 2012 Dec 12.
The complex metabolic networks in plants are highly compartmentalized and biochemical steps of a single pathway can take place in multiple subcellular locations. Our knowledge regarding reactions and precursor compounds in the various cellular compartments has increased in recent years due to innovations in tracking the spatial distribution of proteins and metabolites. Nevertheless, to date only few studies have integrated subcellular localization criteria in metabolic engineering attempts. Here, we highlight the crucial factors for subcellular-localization-based strategies in plant metabolic engineering including substrate availability, enzyme targeting, the role of transporters, and multigene transfer approaches. The availability of compartmentalized metabolic network models for plants in the near future will greatly advance the integration of localization constraints in metabolic engineering experiments and aid in predicting their outcomes.
植物中复杂的代谢网络高度分隔，单一途径的生化步骤可以发生在多个亚细胞位置。近年来，由于追踪蛋白质和代谢物空间分布的创新，我们对各种细胞区室中反应和前体化合物的了解有所增加。然而，迄今为止，只有少数研究将亚细胞定位标准整合到代谢工程尝试中。在这里，我们强调了基于亚细胞定位的策略在植物代谢工程中的关键因素，包括底物可用性、酶靶向、转运蛋白的作用和多基因转移方法。在不久的将来，植物分区代谢网络模型的可用性将极大地促进代谢工程实验中定位约束的整合，并有助于预测其结果。
