Mio M, Akahori H, Sugimoto Y, Akagi M, Tasaka K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Pharmacology. 1989;38(3):191-200. doi: 10.1159/000138537.
The maturational changes in the degree of homologous passive cutaneous anaphylaxis (PCA) and the histamine release from peritoneal mast cells induced by several secretagogues were studied using Wistar rats (4-40 weeks old). Although the increase in vascular permeability of the rat skin induced by intradermal injection of histamine did not change significantly from one maturation period to the next, 6- to 8-weeks old rats were both the most susceptible to PCA reactions and the most responsive to histamine-releasing stimuli. Among rats in this age group (6-8 weeks), the fluidity of the resting cell membrane and the extent of membrane fluidity increase in response to compound 48/80 were greatest. Analysis of the lipid composition of mast cells indicated that the ratio of cholesterol to phospholipids was lowest at the age of 6-8 weeks. From the present study, we concluded that the maturational changes in the extent of histamine release seem to be related to membrane fluidity, which has a profile similar to that of maturation.
利用Wistar大鼠（4 - 40周龄），研究了同源被动皮肤过敏反应（PCA）程度以及几种促分泌剂诱导的腹膜肥大细胞组胺释放的成熟变化。尽管皮内注射组胺诱导的大鼠皮肤血管通透性增加在不同成熟期之间没有显著变化，但6至8周龄的大鼠对PCA反应最敏感，对组胺释放刺激的反应也最强烈。在这个年龄组（6 - 8周）的大鼠中，静息细胞膜的流动性以及对化合物48/80的膜流动性增加程度最大。肥大细胞脂质组成分析表明，胆固醇与磷脂的比例在6 - 8周龄时最低。从本研究中，我们得出结论，组胺释放程度的成熟变化似乎与膜流动性有关，其变化模式与成熟过程相似。
