Chen Yong-Liang, Hu Hang-Wei, Han Hong-Yan, Du Yue, Wan Shi-Qiang, Xu Zhu-Wen, Chen Bao-Dong
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
FEMS Microbiol Ecol. 2014 Jul;89(1):67-79. doi: 10.1111/1574-6941.12336. Epub 2014 Apr 28.
Based on a 6-year field trial in a temperate steppe in Inner Mongolia, we investigated the effects of nitrogen (N) and phosphorus (P) fertilization and mowing on the abundance and community compositions of ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) upon early (May) and peak (August) plant growth using quantitative PCR (qPCR), terminal-restriction fragment length polymorphism (T-RFLP), cloning and sequencing. The results showed that N fertilization changed AOB community composition and increased AOB abundance in both May and August, but significantly decreased AOA abundance in May. By contrast, P fertilization significantly influenced AOB abundance only in August. Mowing significantly decreased AOA abundance and had little effect on AOA community compositions in May, while significantly influencing AOB abundance in both May and August, Moreover, AOA and AOB community structures showed obvious seasonal variations between May and August. Phylogenetic analysis showed that all AOA sequences fell into the Nitrososphaera cluster, and the AOB community was dominated by Nitrosospira Cluster 3. The results suggest that fertilization and mowing play important roles in affecting the abundance and community compositions of AOA and AOB.
基于在内蒙古温带草原进行的为期6年的田间试验，我们利用定量聚合酶链反应（qPCR）、末端限制性片段长度多态性分析（T-RFLP）、克隆和测序技术，研究了氮（N）、磷（P）施肥及割草对植物生长早期（5月）和高峰期（8月）氨氧化细菌（AOB）和氨氧化古菌（AOA）的丰度及群落组成的影响。结果表明，施氮改变了AOB群落组成，且在5月和8月均增加了AOB丰度，但在5月显著降低了AOA丰度。相比之下，施磷仅在8月对AOB丰度有显著影响。割草在5月显著降低了AOA丰度，对AOA群落组成影响较小，而在5月和8月均显著影响AOB丰度。此外，5月至8月期间，AOA和AOB群落结构呈现明显的季节性变化。系统发育分析表明，所有AOA序列均属于亚硝化球菌属（Nitrososphaera）簇，AOB群落以亚硝化螺菌属（Nitrosospira）簇3为主。结果表明，施肥和割草在影响AOA和AOB的丰度及群落组成方面发挥着重要作用。
