Nieri-Bastos Fernanda Aparecida, Lopes Marcos Gomes, Cançado Paulo Henrique Duarte, Rossa Giselle Ayres Razera, Faccini João Luiz Horácio, Gennari Solange Maria, Labruna Marcelo Bahia
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
Embrapa Gado de Corte, Campo Grande, MS, Brasil.
Mem Inst Oswaldo Cruz. 2014 Apr;109(2):259-61. doi: 10.1590/0074-0276140283.
Adult ticks of the species Amblyomma parvum were collected from the vegetation in the Pantanal biome (state of Mato Grosso do Sul) and from horses in the Cerrado biome (state of Piauí) in Brazil. The ticks were individually tested for rickettsial infection via polymerase chain reaction (PCR) targeting three rickettsial genes, gltA, ompA and ompB. Overall, 63.5% (40/63) and 66.7% (2/3) of A. parvum ticks from Pantanal and Cerrado, respectively, contained rickettsial DNA, which were all confirmed by DNA sequencing to be 100% identical to the corresponding fragments of the gltA, ompA and ompB genes of Candidatus Rickettsia andeanae. This report is the first to describe Ca. R. andeanae in Brazil.
微小扇头蜱成虫采自巴西潘塔纳尔生物群落区（南马托格罗索州）的植被以及塞拉多生物群落区（皮奥伊州）的马匹。通过针对三个立克次体基因（gltA、ompA和ompB）的聚合酶链反应（PCR）对蜱虫进行个体立克次体感染检测。总体而言，分别来自潘塔纳尔和塞拉多的微小扇头蜱中，63.5%（40/63）和66.7%（2/3）含有立克次体DNA，经DNA测序证实，这些DNA与“假中间立克次体”gltA、ompA和ompB基因的相应片段100%相同。本报告首次描述了巴西的“假中间立克次体”。
