Holland Aleicia, Duivenvoorden Leo J, Kinnear Susan H W
School of Medical and Applied Sciences, Centre for Environmental Management, Central Queensland University, Rockhampton, Queensland, 4702, Australia,
Ecotoxicology. 2014 Jul;23(5):939-45. doi: 10.1007/s10646-014-1237-3. Epub 2014 Apr 9.
Differences relating to the ability of various types of humic substances (HS) to influence toxicity of pollutants have been reported in the literature, but there still remains a gap in understanding whether various HS will have the same influence on the toxicity of acid mine drainage (AMD). This study investigated differences in the ability of Aldrich humic acid (AHA), Suwannee River humic acid and Suwannee River fulvic acid to decrease toxicity of AMD to the freshwater shrimp (Caridina sp. D). Toxicity tests were conducted over 96 h and used Mount Morgan open pit water as source of AMD and Dee River water as control/diluents. Concentrations of 0-4 % AMD at 0 mg/L HS, 10 mg/L AHA, 10 mg/L Suwannee River humic acid and 10 mg/L Suwannee River fulvic acid were used. Significantly higher survival of shrimp was recorded in the HS treatments compared with the treatment containing no HS. No significant differences were found among HS type. HS considerably increased LC50 values irrespective of type, from 1.29 (0 mg/L HS) to 2.12 % (AHA); 2.19 (Suwannee River humic acid) and 2.22 % (Suwannee River fulvic acid). These results support previous work that HS decrease the toxicity of AMD to freshwater organisms, but with the novel finding that this ability occurs irrespective of HS type. These results increase the stock of knowledge regarding HS and may contribute to a possible remediation option for AMD environments.
文献中已报道了不同类型腐殖质（HS）影响污染物毒性能力的差异，但对于各种HS对酸性矿山排水（AMD）毒性是否具有相同影响的理解仍存在差距。本研究调查了奥尔德里奇腐殖酸（AHA）、苏万尼河腐殖酸和苏万尼河富里酸降低AMD对淡水虾（Caridina sp. D）毒性能力的差异。毒性试验持续96小时，使用摩根山露天矿坑水作为AMD的来源，迪河水作为对照/稀释剂。使用了0 mg/L HS、10 mg/L AHA、10 mg/L苏万尼河腐殖酸和10 mg/L苏万尼河富里酸时0 - 4% AMD的浓度。与不含HS的处理相比，HS处理中记录到虾的存活率显著更高。在HS类型之间未发现显著差异。无论类型如何，HS都显著提高了半数致死浓度（LC50）值，从1.29（0 mg/L HS）提高到2.12%（AHA）；2.19（苏万尼河腐殖酸）和2.22%（苏万尼河富里酸）。这些结果支持了之前关于HS降低AMD对淡水生物毒性的研究工作，但有一个新发现，即这种能力与HS类型无关。这些结果增加了关于HS的知识储备，并可能为AMD环境提供一种可能的修复选择。
