Gardian Zdenko, Litvín Radek, Bína David, Vácha František
Institute of Plant Molecular Biology, Biology Centre ASCR, Branisovska 31, 37005, Ceske Budejovice, Czech Republic.
Photosynth Res. 2014 Jul;121(1):79-86. doi: 10.1007/s11120-014-9998-3. Epub 2014 Apr 9.
Fucoxanthin-chlorophyll proteins (FCP) are the major light-harvesting proteins of diatom algae, a major contributor to marine carbon fixation. FCP complexes from representatives of centric (Cyclotella meneghiniana) and pennate (Phaeodactylum tricornutum) diatoms were prepared by sucrose gradient centrifugation and studied by means of electron microscopy followed by single particle analysis. The oligomeric FCP from a centric diatom were observed to take the form of unusual chain-like or circular shapes, a very unique supramolecular assembly for such antennas. The existence of the often disputed oligomeric form of FCP in pennate diatoms has been confirmed. Contrary to the centric diatom FCP, pennate diatom FCP oligomers are very similar to oligomeric antennas from related heterokont (Stramenopila) algae. Evolutionary aspects of the presence of novel light-harvesting protein arrangement in centric diatoms are discussed.
岩藻黄质 - 叶绿素蛋白（FCP）是硅藻的主要捕光蛋白，硅藻是海洋碳固定的主要贡献者。通过蔗糖梯度离心法制备了来自中心硅藻（小环藻）和羽纹硅藻（三角褐指藻）代表种的FCP复合物，并通过电子显微镜和单颗粒分析进行了研究。观察到来自中心硅藻的寡聚FCP呈不寻常的链状或圆形，这对于此类天线来说是非常独特的超分子组装形式。已经证实了羽纹硅藻中经常存在争议的FCP寡聚体形式。与中心硅藻FCP相反，羽纹硅藻FCP寡聚体与相关不等鞭毛藻（硅藻门）藻类的寡聚天线非常相似。本文讨论了中心硅藻中新型捕光蛋白排列存在的进化方面。
