Martinotti M G
Istituto di Microbiologia, Università di Torino.
G Batteriol Virol Immunol. 1987 Jan-Dec;80(1-12):267-76.
With the recent and rapid developments in recombinant DNA technology it has become much easier to use nucleic acid probes in diagnosis of infectious diseases. In this review some parameters like designing of probes, selection of tags, hybridization reaction conditions and in situ hybridization have been discussed. In addition, some examples have been reported which employ techniques of dot-blot hybridization and in situ hybridization for the diagnosis of latent cytomegalovirus infection.
随着重组DNA技术最近的迅速发展,使用核酸探针诊断传染病变得容易得多。在这篇综述中,讨论了一些参数,如探针的设计、标记的选择、杂交反应条件和原位杂交。此外,还报道了一些使用斑点印迹杂交和原位杂交技术诊断潜伏性巨细胞病毒感染的例子。