在构巢曲霉中，一大簇高表达基因对于生长和发育是可有可无的。
A large cluster of highly expressed genes is dispensable for growth and development in Aspergillus nidulans.
作者信息
Aramayo R, Adams T H, Timberlake W E
机构信息
Department of Genetics, University of Georgia, Athens 30602.
出版信息
Genetics. 1989 May;122(1):65-71. doi: 10.1093/genetics/122.1.65.
Abstract
We investigated the functions of the highly expressed, sporulation-specific SpoC1 genes of Aspergillus nidulans by deleting the entire 38-kb SpoC1 gene cluster. The resultant mutant strain did not differ from the wild type in (1) growth rate, (2) morphology of specialized reproductive structures formed during completion of the asexual or sexual life cycles, (3) sporulation efficiency, (4) spore viability or (5) spore resistance to environmental stress. Thus, deletion of the SpoC1 gene cluster, representing 0.15% of the A. nidulans genome, had no readily detectable phenotypic effects. Implications of this result are discussed in the context of major alterations in gene expression that occur during A. nidulans development.
摘要
我们通过删除整个38 kb的SpoC1基因簇，研究了构巢曲霉中高度表达的、孢子形成特异性的SpoC1基因的功能。所得突变菌株在以下方面与野生型没有差异：(1)生长速率，(2)在无性或有性生活周期完成过程中形成的特殊生殖结构的形态，(3)孢子形成效率，(4)孢子活力或(5)孢子对环境压力的抗性。因此，代表构巢曲霉基因组0.15%的SpoC1基因簇的缺失没有产生易于检测到的表型效应。我们在构巢曲霉发育过程中发生的基因表达重大变化的背景下讨论了这一结果的意义。
相似文献
1
A large cluster of highly expressed genes is dispensable for growth and development in Aspergillus nidulans.在构巢曲霉中，一大簇高表达基因对于生长和发育是可有可无的。
Genetics. 1989 May;122(1):65-71. doi: 10.1093/genetics/122.1.65.
2
Structure and regulated expression of the SpoC1 gene cluster from Aspergillus nidulans.构巢曲霉SpoC1基因簇的结构与调控表达
J Mol Biol. 1984 Nov 25;180(1):91-109. doi: 10.1016/0022-2836(84)90432-7.
3
Position-dependent and -independent mechanisms regulate cell-specific expression of the SpoC1 gene cluster of Aspergillus nidulans.位置依赖性和非依赖性机制调控构巢曲霉SpoC1基因簇的细胞特异性表达。
Mol Cell Biol. 1987 Jan;7(1):427-34. doi: 10.1128/mcb.7.1.427-434.1987.
4
Transcriptomic, Protein-DNA Interaction, and Metabolomic Studies of VosA, VelB, and WetA in Aspergillus nidulans Asexual Spores.转录组学、蛋白质-DNA 相互作用和 Aspergillus nidulans 无性孢子中 VosA、VelB 和 WetA 的代谢组学研究。
mBio. 2021 Feb 9;12(1):e03128-20. doi: 10.1128/mBio.03128-20.
5
The nsdD gene encodes a putative GATA-type transcription factor necessary for sexual development of Aspergillus nidulans.nsdD基因编码一种推定的GATA型转录因子，该转录因子是构巢曲霉有性发育所必需的。
Mol Microbiol. 2001 Jul;41(2):299-309. doi: 10.1046/j.1365-2958.2001.02472.x.
6
Clustering of spore-specific genes in Aspergillus nidulans.构巢曲霉中孢子特异性基因的聚类
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5976-80. doi: 10.1073/pnas.79.19.5976.
7
Cellular effects of misscheduled brlA, abaA, and wetA expression in Aspergillus nidulans.构巢曲霉中brlA、abaA和wetA表达失调的细胞效应。
Can J Microbiol. 1994 Dec;40(12):1035-42. doi: 10.1139/m94-164.
8
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans.构巢曲霉无性孢子中特异性表达的基因簇的组织。
Cell. 1981 Oct;26(1 Pt 1):29-37. doi: 10.1016/0092-8674(81)90030-1.
9
Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development.flbA是构巢曲霉无性孢子形成的早期调节因子，其过表达会导致brlA激活和发育的过早启动。
Mol Microbiol. 1994 Oct;14(2):323-34. doi: 10.1111/j.1365-2958.1994.tb01293.x.
10
Response regulators SrrA and SskA are central components of a phosphorelay system involved in stress signal transduction and asexual sporulation in Aspergillus nidulans.应答调节因子SrrA和SskA是构巢曲霉中参与应激信号转导和无性孢子形成的磷酸化中继系统的核心组成部分。
Eukaryot Cell. 2007 Sep;6(9):1570-83. doi: 10.1128/EC.00085-07. Epub 2007 Jul 13.
引用本文的文献
1
LaeA Controls Citric Acid Production through Regulation of the Citrate Exporter-Encoding Gene in Aspergillus luchuensis mut. .LaeA 通过调控 Aspergillus luchuensis mut. 中柠檬酸外排基因来控制柠檬酸的产生。
Appl Environ Microbiol. 2020 Feb 18;86(5). doi: 10.1128/AEM.01950-19.
2
Mitochondrial Citrate Transporters CtpA and YhmA Are Required for Extracellular Citric Acid Accumulation and Contribute to Cytosolic Acetyl Coenzyme A Generation in Aspergillus luchuensis mut. .线粒体柠檬酸转运蛋白 CtpA 和 YhmA 是细胞外柠檬酸积累所必需的，有助于 Aspergillus luchuensis mut.. 细胞质乙酰辅酶 A 的生成。
Appl Environ Microbiol. 2019 Apr 4;85(8). doi: 10.1128/AEM.03136-18. Print 2019 Apr 15.
3
GfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus.GfsA 编码了一种新型半乳呋喃糖基转移酶，参与了 Aspergillus nidulans 和 Aspergillus fumigatus 中 O-聚糖半乳呋喃糖抗原的生物合成。
Mol Microbiol. 2013 Dec;90(5):1054-1073. doi: 10.1111/mmi.12416. Epub 2013 Oct 21.
4
Regulation of conidiation by light in Aspergillus nidulans.在粗糙脉孢菌中，光照对分生孢子形成的调控。
Genetics. 2011 Aug;188(4):809-22. doi: 10.1534/genetics.111.130096. Epub 2011 May 30.
5
Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans.蛋白质O-甘露糖基转移酶B和C支持构巢曲霉的菌丝发育和分化。
Eukaryot Cell. 2009 Oct;8(10):1465-74. doi: 10.1128/EC.00371-08. Epub 2009 Jul 31.
6
The Aspergillus nidulans kinesin-3 UncA motor moves vesicles along a subpopulation of microtubules.构巢曲霉驱动蛋白-3 UncA分子马达沿着微管亚群移动囊泡。
Mol Biol Cell. 2009 Jan;20(2):673-84. doi: 10.1091/mbc.e08-07-0685. Epub 2008 Nov 26.
7
Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination.通过环出重组在米曲霉和酱油曲霉等曲霉菌中产生大的染色体缺失
Appl Environ Microbiol. 2008 Dec;74(24):7684-93. doi: 10.1128/AEM.00692-08. Epub 2008 Oct 24.
8
A cytosine methyltransferase homologue is essential for sexual development in Aspergillus nidulans.一种胞嘧啶甲基转移酶同源物对构巢曲霉的有性发育至关重要。
PLoS One. 2008 Jun 25;3(6):e2531. doi: 10.1371/journal.pone.0002531.
9
The role of the kinesin motor KipA in microtubule organization and polarized growth of Aspergillus nidulans.驱动蛋白KipA在构巢曲霉微管组织和极性生长中的作用。
Mol Biol Cell. 2005 Feb;16(2):497-506. doi: 10.1091/mbc.e04-02-0083. Epub 2004 Nov 24.
10
The Zn(II)2Cys6 putative Aspergillus nidulans transcription factor repressor of sexual development inhibits sexual development under low-carbon conditions and in submersed culture.构巢曲霉中假定的参与性发育的Zn(II)2Cys6转录因子阻遏物在低碳条件下和深层培养时会抑制性发育。
Genetics. 2005 Feb;169(2):619-30. doi: 10.1534/genetics.104.030767. Epub 2004 Nov 1.
本文引用的文献
1
The genetics of Aspergillus nidulans.构巢曲霉的遗传学
Adv Genet. 1953;5:141-238. doi: 10.1016/s0065-2660(08)60408-3.
2
Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans.丝状真菌构巢曲霉中奎尼酸分解代谢的遗传学和生物化学方面
Biochem Genet. 1982 Apr;20(3-4):271-86. doi: 10.1007/BF00484424.
3
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans.构巢曲霉无性孢子中特异性表达的基因簇的组织。
Cell. 1981 Oct;26(1 Pt 1):29-37. doi: 10.1016/0092-8674(81)90030-1.
4
Developmental gene regulation in Aspergillus nidulans.构巢曲霉中的发育基因调控。
Dev Biol. 1980 Aug;78(2):497-510. doi: 10.1016/0012-1606(80)90349-8.
5
Temperature-shift analysis of conidial development in Aspergillus nidulans.构巢曲霉分生孢子发育的温度转换分析
Dev Biol. 1982 Sep;93(1):92-103. doi: 10.1016/0012-1606(82)90242-1.
6
Clustering of spore-specific genes in Aspergillus nidulans.构巢曲霉中孢子特异性基因的聚类
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5976-80. doi: 10.1073/pnas.79.19.5976.
7
Structure and regulated expression of the SpoC1 gene cluster from Aspergillus nidulans.构巢曲霉SpoC1基因簇的结构与调控表达
J Mol Biol. 1984 Nov 25;180(1):91-109. doi: 10.1016/0022-2836(84)90432-7.
8
Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans.构巢曲霉鸟氨酸氨甲酰基转移酶基因的克隆与特性分析
Gene. 1983 Nov;25(1):109-17. doi: 10.1016/0378-1119(83)90173-7.
9
The genetic location of three mutations impairing penicillin production in Aspergillus nidulans.构巢曲霉中三个影响青霉素产生的突变的基因定位。
J Gen Microbiol. 1983 Oct;129(10):3027-33. doi: 10.1099/00221287-129-10-3027.
10
Transformation of Aspergillus nidulans by using a trpC plasmid.利用trpC质粒对构巢曲霉进行转化。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470-4. doi: 10.1073/pnas.81.5.1470.
Zotero 插件