Yang H-L, Huang J, Yang B, Liu F, Zhang Q-L
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Shanghai Ocean University, Shanghai, China; National Laboratory for Marine Science and Technology, Qingdao, China.
Lett Appl Microbiol. 2014 Aug;59(2):200-6. doi: 10.1111/lam.12267. Epub 2014 May 2.
White spot syndrome virus (WSSV) is one of the main pathogens seriously threatening penaeid shrimp farming in the world. This study designed and synthesized five specific primers based on the conserved sequences of WSSV genome, optimized the reaction system and conditions, and finally established a cross-priming amplification (CPA)-based detection method for WSSV (WSSV-CPA). The results indicated that the optimized reaction temperature and time of WSSV-CPA were 63°C and 60 min, respectively. The detection limit of the WSSV-CPA assay was as low as 10 copies μl(-1) and shared same sensitivity with the WSSV-qPCR assay. Due to dispensing with expensive thermal cycler, time- and cost-saving, and ease of use in field, it is anticipated that the WSSV-CPA method developed in this study will be instrumental for the diagnosis and surveillance of WSSV.
White spot syndrome, caused by white spot syndrome virus (WSSV), is the major disease threatening the shrimp aquaculture industry and leads to tens of billion dollars of economic losses in the world each year. This study established a CPA-based method for detecting WSSV, which is rapid, sensitive and specific. It is anticipated that this novel assay will be instrumental for diagnosis and surveillance of WSSV.
白斑综合征病毒（WSSV）是严重威胁全球对虾养殖的主要病原体之一。本研究基于WSSV基因组的保守序列设计并合成了5条特异性引物，优化了反应体系和条件，最终建立了基于交叉引物扩增（CPA）的WSSV检测方法（WSSV-CPA）。结果表明，WSSV-CPA优化后的反应温度和时间分别为63℃和60分钟。WSSV-CPA检测方法的检测限低至10拷贝μl（-1），与WSSV-qPCR检测方法具有相同的灵敏度。由于无需昂贵的热循环仪，省时省钱且便于现场使用，预计本研究开发的WSSV-CPA方法将有助于WSSV的诊断和监测。
由白斑综合征病毒（WSSV）引起的白斑综合征是威胁对虾养殖业的主要疾病，每年在全球造成数百亿美元的经济损失。本研究建立了一种基于CPA的WSSV检测方法，该方法快速、灵敏且特异。预计这种新的检测方法将有助于WSSV的诊断和监测。
