Philippe E, Droz B
Institut d'Histologie et d'Embryologie, Faculté de Médecine, Université de Lausanne, Switzerland.
J Comp Neurol. 1989 May 1;283(1):153-60. doi: 10.1002/cne.902830113.
In the chicken dorsal root ganglia, two neuronal subpopulations referred to as A1 and B1 share in common an immunoreactivity to antisera raised to calbindin D-28k but are distinguished by their cytological and ultrastructural characteristics. To determine the peripheral targets innervated by calbindin-immunoreactive neurons in lumbosacral dorsal root ganglia, cryostat sections of various hindlimb tissues were treated with anticalbindin antisera. Calbindin-immunostained axons were clearly detected in skeletal muscle. Large myelinated nerve fibres and afferent axon terminals in neuromuscular spindles were calbindin-immunoreactive; thin unmyelinated nerve fibres were also immunostained in nerve bundles of the perimysium. Since motoneurons and neurons of the autonomic nervous system were devoid of calbindin immunostaining, it was suggested that the immunoreactive axons found in skeletal muscle originate from sensory neurons expressing a calbindin immunoreaction in the dorsal root ganglia. This hypothesis was corroborated after introduction of wheat germ agglutinin coupled with horseradish peroxidase or colloidal gold particles into the sartorius muscle. The retrogradely transported tracer was collected only in ganglion cell bodies which displayed the ultrastructural characteristics of A1 and B1 sensory neurons. On the basis of calbindin immunoreaction and of tracer retrograde transport, it is concluded that ganglion cells of subclasses A1 and B1 contribute to the sensory innervation of skeletal muscle in the chicken.
在鸡的背根神经节中，有两个神经元亚群，即A1和B1，它们对针对钙结合蛋白D - 28k产生的抗血清具有共同的免疫反应性，但在细胞学和超微结构特征上有所区别。为了确定腰骶部背根神经节中钙结合蛋白免疫反应性神经元所支配的外周靶标，对各种后肢组织的冰冻切片用抗钙结合蛋白抗血清进行处理。在骨骼肌中清晰地检测到了钙结合蛋白免疫染色的轴突。神经肌肉纺锤体中的大型有髓神经纤维和传入轴突终末是钙结合蛋白免疫反应性的；在肌束膜的神经束中，细的无髓神经纤维也被免疫染色。由于运动神经元和自主神经系统的神经元没有钙结合蛋白免疫染色，因此有人提出，在骨骼肌中发现的免疫反应性轴突起源于背根神经节中表达钙结合蛋白免疫反应的感觉神经元。在用小麦胚凝集素与辣根过氧化物酶或胶体金颗粒结合物注入缝匠肌后，这一假设得到了证实。逆行运输的示踪剂仅在显示出A1和B1感觉神经元超微结构特征的神经节细胞体中收集到。基于钙结合蛋白免疫反应和示踪剂逆行运输，得出结论：A1和B1亚类的神经节细胞参与了鸡骨骼肌的感觉神经支配。
