Kuramoto H, Kuwano R
Department of Anatomy, Yamanashi Medical University, Japan.
Cell Tissue Res. 1994 Oct;278(1):57-64. doi: 10.1007/BF00305778.
Immunoreactivity for calbindin was found in nerve endings with irregular laminar shapes in the rat esophagus. In the myenteric ganglia, laminar endings of a range of sizes formed a complex network and appeared to lie at the surface of the ganglion. The myenteric ganglia that contained nerve endings were most abundant in the upper portion of the esophagus, their number decreasing orally to anally. Calbindin-immunoreactive nerve cell bodies were scattered throughout the esophagus. Laminar terminals were found in the connective tissue of the lamina propria immediately beneath the epithelium and in the muscularis mucosae. Occasional nerve branches formed a network of aborizing endings that surrounded part of the submucosal arterioles. Immunoreactive nerve endings in the mucosa and submucosa were present only in the upper part of the cervical esophagus. Unilateral vagotomy caused a remarkable decrease in the number of the myenteric ganglia containing the calbindin-immunoreactive laminar endings after 15 days or survival; in some of ganglia, the laminar structures disappeared and nerve endings showing weak immunoreactivity had an indistinct appearance, so that the outline of the ganglia became obscure. In operated rats at 24 days, the number of innervated ganglia was about half that in normal rats. However, there was no change in the morphology and the occurrence of the immunoreactive laminar structures in the mucosa and submucosa after denervation. The results show that many of the laminar endings that are immunoreactive for calbindin in the myenteric ganglia are derived from the vagus nerve. Thus, the calbindin-immunoreactive nerve endings with laminar expansions that are found in the rat esophageal wall could be sensory receptors.
在大鼠食管中，钙结合蛋白免疫反应性存在于形状不规则的层状神经末梢中。在肌间神经节中，一系列大小的层状末梢形成复杂网络，似乎位于神经节表面。含有神经末梢的肌间神经节在食管上部最为丰富，其数量从口端到肛端逐渐减少。钙结合蛋白免疫反应性神经细胞体散布于整个食管。层状终末见于上皮下方固有层的结缔组织以及黏膜肌层。偶尔有神经分支形成分支状终末网络，围绕部分黏膜下小动脉。黏膜和黏膜下层的免疫反应性神经末梢仅存在于颈段食管上部。单侧迷走神经切断术后15天或存活期后，含有钙结合蛋白免疫反应性层状终末的肌间神经节数量显著减少；在一些神经节中，层状结构消失，免疫反应性较弱的神经末梢外观模糊，神经节轮廓变得不清晰。在术后24天的大鼠中，有神经支配的神经节数量约为正常大鼠的一半。然而，去神经支配后黏膜和黏膜下层免疫反应性层状结构的形态和出现情况没有变化。结果表明，肌间神经节中许多对钙结合蛋白有免疫反应性的层状终末源自迷走神经。因此，在大鼠食管壁中发现的具有层状扩张的钙结合蛋白免疫反应性神经末梢可能是感觉感受器。
