Bo Lin, O'Mahony Christy Charlton, Semenova Yuliya, Gilmartin Niamh, Wang Pengfei, Farrell Gerald
Opt Express. 2014 Apr 7;22(7):8150-5. doi: 10.1364/OE.22.008150.
Optical microfibers and related structures which incorporate large evanescent field and minimal size offer new opportunities for biosensing applications. In this paper we report the development of an immunosensor based on a tapered microfiber coupler embedded in a low refractive index polymer. Biomolecules adsorbed on the microfiber coupler surface modify the surrounding refractive index. By immobilizing antigens on the surface of the sensing area, the microfiber coupler was able to operate as a label-free immunosensor to detect specific antibodies. We experimentally demonstrated for the first time the sensing ability of this sensor using a fibrinogen antigen-antibody pair. By monitoring the spectral shift in the wavelength domain, the sensor was shown to be capable of detecting the specific binding between fibrinogen and anti-fibrinogen. The detected signal was found to be proportional to the anti-fibrinogen present.
具有大倏逝场和最小尺寸的光学微纤维及相关结构为生物传感应用提供了新机遇。在本文中，我们报告了一种基于嵌入低折射率聚合物中的锥形微纤维耦合器的免疫传感器的研制情况。吸附在微纤维耦合器表面的生物分子会改变周围的折射率。通过将抗原固定在传感区域表面，微纤维耦合器能够作为无标记免疫传感器来检测特定抗体。我们首次通过实验证明了使用纤维蛋白原抗原 - 抗体对时该传感器的传感能力。通过监测波长域中的光谱位移，该传感器能够检测纤维蛋白原与抗纤维蛋白原之间的特异性结合。检测到的信号与存在的抗纤维蛋白原成正比。
