嗜热四膜虫SerH3表面蛋白编码cDNA的鉴定。
Identification of a cDNA coding for the SerH3 surface protein of Tetrahymena thermophila.
作者信息
Doerder F P, Hallberg R L
机构信息
Department of Biology, Cleveland State University, Ohio 44115.
出版信息
J Protozool. 1989 May-Jun;36(3):304-7. doi: 10.1111/j.1550-7408.1989.tb05368.x.
We have identified a Tetrahymena thermophila cDNA-containing plasmid (pC6) which hybridizes to a 1.47-kB RNA whose changes in cellular concentration parallel the changes in synthetic rate of a major cell surface protein. From a molecular and genetic analysis of strains expressing the gene (SerH3) encoding this protein, and of strains expressing immunologically distinct alleles of this gene, we conclude that pC6 encodes a portion of the SerH3 allele.
我们鉴定出了一种含有嗜热四膜虫cDNA的质粒（pC6），它能与一段1.47千碱基的RNA杂交，该RNA在细胞内的浓度变化与一种主要细胞表面蛋白的合成速率变化平行。通过对表达编码该蛋白的基因（SerH3）的菌株以及表达该基因免疫特性不同的等位基因的菌株进行分子和遗传分析，我们得出结论：pC6编码SerH3等位基因的一部分。
Zotero 插件