Guo Jun-Chao, Li Jian, Zhao Yu-Pei, Zhou Li, Cui Quan-Cai, Zhou Wei-Xun, Zhang Tai-Ping, You Lei, Shu Hong
Department of General Surgery, Peking Union Medical College Hospital, National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, 100730, China.
World J Surg. 2014 Aug;38(8):2126-31. doi: 10.1007/s00268-014-2500-8.
Pancreatic ductal adenocarcinoma (PDAC) has long been acknowledged to have a dismal prognosis. Therefore, prognostic markers, especially molecular ones, are of interest. So far, expression of Neural Wiskott-Aldrich syndrome protein (N-WASP) and its associations with clinicopathologic variables and prognosis for patients with PDAC remain unknown.
N-WASP expression was detected by immunohistochemical staining in a tissue microarray consisted of tumor and nontumor samples from 86 patients with PDAC. The correlations of N-WASP expression with clinicopathologic features and overall survival were evaluated. In addition, risk factors of perineural invasion (PNI) were identified.
High expression of N-WASP was more frequent in tumor than in nontumor tissues of PDAC patients (45.3 vs. 19.8%, p < 0.001). The rank of N-WASP grading was significantly higher in tumor tissues than in nontumor tissues (p = 0.048). Also, high expression of N-WASP in tumor tissues was significantly associated with PNI, and lymph node status had a marginally significant relation to tumoral N-WASP expression. Univariate analyses showed that, in addition to conventional clinicopathologic variables, including sex, histologic grade, PNI and lymph node metastasis, high tumoral N-WASP expression was an independent marker of PNI and served as a significant predictor of poor overall survival. The prognostic implication of N-WASP expression was not proven In the multivariate analysis.
Our data showed highly up-regulated expression of N-WASP in PDAC tissues, its correlations with PNI, and its association with an unfavorable prognosis.
长期以来，胰腺导管腺癌（PDAC）的预后一直很差。因此，预后标志物，尤其是分子标志物，备受关注。到目前为止，神经威斯科特-奥尔德里奇综合征蛋白（N-WASP）的表达及其与PDAC患者临床病理变量和预后的关系尚不清楚。
通过免疫组织化学染色在一个组织芯片中检测N-WASP的表达，该组织芯片由86例PDAC患者的肿瘤和非肿瘤样本组成。评估N-WASP表达与临床病理特征及总生存期的相关性。此外，确定神经周围侵犯（PNI）的危险因素。
PDAC患者肿瘤组织中N-WASP高表达的频率高于非肿瘤组织（45.3%对19.8%，p<0.001）。肿瘤组织中N-WASP分级的排名显著高于非肿瘤组织（p=0.048）。此外，肿瘤组织中N-WASP的高表达与PNI显著相关，淋巴结状态与肿瘤N-WASP表达有边缘显著关系。单因素分析表明，除了传统的临床病理变量，包括性别、组织学分级、PNI和淋巴结转移外，肿瘤N-WASP高表达是PNI的独立标志物，也是总生存期差的重要预测指标。在多因素分析中，N-WASP表达的预后意义未得到证实。
我们的数据显示PDAC组织中N-WASP表达高度上调，其与PNI相关，且与不良预后相关。
