Lin C P, Böhnke M, Draeger J
Department of Ophthalmology, Kaohsiung Medical College, Taiwan, ROC.
Ophthalmic Res. 1989;21(2):97-100. doi: 10.1159/000266785.
A model to induce scattered cell damage in the corneal endothelium was set up by shaking a pig cornea in a glass bottle with 0.18% NaCl. Disseminated endothelial-cell losses, whose amounts correlated with the durations of treatment, were observed in the corneas which were shaken for less than 8 min. Following organ culturing for 1 week, most of the corneas still possessed intact endothelia which means that even after the treatment the endothelium still keeps a normal regenerative ability. With this method of design, a good model is provided to study the regeneration of the corneal endothelium.
通过在装有0.18%氯化钠的玻璃瓶中摇晃猪角膜，建立了一种诱导角膜内皮细胞散在性损伤的模型。在摇晃时间少于8分钟的角膜中观察到散在性内皮细胞丢失，其数量与处理时间相关。器官培养1周后，大多数角膜的内皮仍然完整，这意味着即使经过处理，内皮仍保持正常的再生能力。通过这种设计方法，为研究角膜内皮的再生提供了一个良好的模型。
