He Fei, Grimes Jeff, Alcaine Samuel D, Nugen Sam R
246 Chenoweth,102 Holdsworth Way, Amherst, MA, USA.
Analyst. 2014 Jun 21;139(12):3002-8. doi: 10.1039/c3an01516e.
Sequential fluid delivery with minimized external equipment is vital towards a point-of-care diagnostic device. In this work, we have further developed the On-chip Electrowetting Valves concept for the sequential delivery of the reagents to the reaction site in a miniaturized capillary-driven microfluidic chip. Specifically, a disposable polymeric microfluidic device was developed containing capillary force driven microchannels. The device was fabricated using laser ablation and inkjet printing and required no external pumping equipment. The assay was conducted on the microchip containing microfluidic channels with embedded electrowetting valves and a porous membrane patterned with capture molecules and colloidal gold labels. To conduct the assay, the microchip was connected with a low voltage supply which was capable of sequentially opening the valves, delivering the sample and the rinsing reagent to generate visual results. Using T7 bacteriophage as a model, we have demonstrated the development of the device, operation of the valves and execution of the automated assay.
使用最少的外部设备进行连续流体输送对于即时诊断设备至关重要。在这项工作中，我们进一步开发了片上电润湿阀概念，用于在小型化的毛细管驱动微流控芯片中将试剂连续输送到反应位点。具体而言，开发了一种一次性聚合物微流控装置，其中包含毛细管力驱动的微通道。该装置采用激光烧蚀和喷墨打印制造，无需外部泵送设备。该检测在含有微流控通道的微芯片上进行，微流控通道中嵌入了电润湿阀以及用捕获分子和胶体金标记物图案化的多孔膜。为了进行检测，将微芯片与能够顺序打开阀门、输送样品和冲洗试剂以产生视觉结果的低压电源相连。以T7噬菌体为模型，我们展示了该装置的开发、阀门的操作以及自动化检测的执行。
