睾酮和雌二醇对雌性大鼠应激诱导的肾上腺和海马体重量变化的影响。

Effects of testosterone and estradiol on stress-induced adrenal and hippocampal weight changes in female rats.

机构信息

Department of Pharmacology, Medical School, National & Kapodistrian University of Athens, Athens, Greece.

Department of Biology & Center for Tissue Regeneration and Engineering (TREND), University of Dayton, Dayton, USA.

出版信息

Hormones (Athens). 2014 Jan-Mar;13(1):119-30. doi: 10.1007/BF03401327.

DOI:10.1007/BF03401327

PMID:24722133

Abstract

OBJECTIVE

To examine the impact of circulating testosterone (T) and the T/Estradiol (T/Ediol) ratio on chronic stress-induced changes of adrenal and hippocampal weight during proestrus (PE) and estrus (E) in female rats.

DESIGN

Stress was composed of repeated vaginal smear screening (VSS) and measured by the emotional reactivity score (ERS). Adrenal and hippocampal weight and the T, Ediol and T/Ediol ratio were assessed in PE and E controls as well as 20 h after sham or left adrenalectomy performed on diestrus-2 (DE-2) and PE, respectively. T was measured in ovariectomized (OVX) rats treated with estradiol benzoate (EB) or vehicle (VEH) and in non-OVX EB-treated rats.

RESULTS

In OVX rats EB treatment increased adrenal weight and T levels. After separation of VEH- and EB-treated rats into the low and high T-range (below and above the mean, respectively), it was observed that higher T was accompanied by higher adrenal weight in EB- compared to VEH-treated rats only in the low T-range. Non-OVX EB-treated rats with high T had lower adrenal weight compared to low T. Cycling rats assigned to the high T-range presented higher T/Ediol ratio but similar ERS and Ediol levels compared to rats in the low T-range, and were characterized by reduced adrenal weight, higher hippocampal weight and prevalence of PE versus E.

CONCLUSIONS

High T and high T/Ediol ratios are prominent in PE compared to E and exert a protective effect on hippocampal neuronal degeneration after similar chronic stress through T-mediated lessening of stress response thus counteracting the stress-promoting effects of Ediol.

摘要

目的

研究循环睾酮（T）和 T/雌二醇（T/Ediol）比值对雌性大鼠发情前期（PE）和发情期（E）慢性应激诱导的肾上腺和海马体重量变化的影响。

设计

应激由重复阴道涂片检查（VSS）组成，并通过情绪反应评分（ERS）进行测量。在 PE 和 E 对照组中评估了肾上腺和海马体重量以及 T、Ediol 和 T/Ediol 比值，同时在分别于 DE-2 和 PE 对去势-2 （DE-2）进行假手术或左肾上腺切除术 20 小时后评估了这些值。在接受苯甲酸雌二醇（EB）或载体（VEH）处理的卵巢切除（OVX）大鼠以及未接受 OVX 的 EB 处理大鼠中测量了 T。

结果

在 OVX 大鼠中，EB 处理增加了肾上腺重量和 T 水平。将 VEH 和 EB 处理的大鼠分为低 T 范围（低于平均值）和高 T 范围（高于平均值）后，观察到仅在低 T 范围内，EB 处理的大鼠中，较高的 T 与较高的肾上腺重量相关。与低 T 大鼠相比，高 T 的非 OVX EB 处理大鼠的肾上腺重量较低。具有高 T 的循环大鼠与低 T 大鼠相比具有更高的 T/Ediol 比值，但 ERS 和 Ediol 水平相似，其特征是肾上腺重量降低，海马体重量增加，PE 期与 E 期的比例更高。