Lemos M, Dumont J E, Erneux C
Institut de Recherche Interdisciplinaire (IRIBHN), Faculté de Médecine, Université Libre de Bruxelles, Belgium.
FEBS Lett. 1989 Jun 5;249(2):321-3. doi: 10.1016/0014-5793(89)80650-7.
Ins(1,4,5)P3 5-phosphatase catalyzes the dephosphorylation of Ins(1,4,5)P3 in the 5-position. In a high speed soluble fraction of bovine brain, there are two soluble 5-phosphatases: type I and type II. The purified Ins(1,4,5)P3 5-phosphatase type I exhibits a major silver-stained band of 43 kDa on denaturing (SDS) gels. It is possible to extract the 5-phosphatase activity form a duplicate lane after gel electrophoresis. The 43 kDa region contains the extractable Ins(1,4,5)P3 5-phosphatase activity.
