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Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate.

作者信息

Celestino Jessyca Dos Reis, Duarte Ana Caroline, Silva Cláudia Maria de Melo, Sena Hellen Holanda, Ferreira Maria do Perpétuo Socorro Borges Carriço, Mallmann Neila Hiraishi, Lima Natacha Pinheiro Costa, Tavares Chanderlei de Castro, de Souza Rodrigo Otávio Silva, Souza Erica Simplício, Souza João Vicente Braga

机构信息

Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.

Instituto Nacional de Pesquisas da Amazônia, INPA, Laboratório de Microbiologia Médica, Manaus, AM, Brazil.

出版信息

Enzyme Res. 2014;2014:725651. doi: 10.1155/2014/725651. Epub 2014 Mar 2.

DOI:10.1155/2014/725651
PMID:24724017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3958758/
Abstract

The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6487/3958758/3823104e5c2e/ER2014-725651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6487/3958758/9e8c2f331ad9/ER2014-725651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6487/3958758/3823104e5c2e/ER2014-725651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6487/3958758/9e8c2f331ad9/ER2014-725651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6487/3958758/3823104e5c2e/ER2014-725651.002.jpg

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