Birmpa Angeliki, Vantarakis Apostolos, Paparrodopoulos Spyros, Whyte Paul, Lyng James
Environmental Microbiology Unit, Department of Public Health, Medical School, University of Patras, Rio, 26500 Patras, Greece.
School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
Biomed Res Int. 2014;2014:673939. doi: 10.1155/2014/673939. Epub 2014 Mar 4.
The aim of the current study was to evaluate the effectiveness of three nonthermal light technologies (NUV-Vis, continuous UV, and HILP) on their ability to inactivate Escherichia coli K12 and Listeria innocua. E. coli K12 was selected as a representative microorganism for the enterohaemorrhagic foodborne pathogen E. coli O157:H7 and L. innocua as a surrogate microorganism for the common foodborne pathogen Listeria monocytogenes, respectively. The liquid matrix used for the disinfection experiments was a liquid matrix (MRD solution). The results of the present study show that the HILP treatment inactivated both E. coli and L. innocua more rapidly and effectively than either continuous UV-C or NUV-vis treatment. With HILP at 2.5 cm from the lamp, E. coli and L. innocua populations were reduced by 3.07 and 3.77 log10 CFU/mL, respectively, after a 5 sec treatment time, and were shown to be below the limit of detection (<0.22 log10 CFU/mL) following 30 sec exposure to HILP (106.2 J/cm(2)). These studies demonstrate the bactericidal efficacy of alternative nonthermal light technologies and their potential as decontamination strategies in the food industry.
本研究的目的是评估三种非热光技术（近紫外 - 可见光、连续紫外线和高强度光脉冲）对灭活大肠杆菌K12和无害李斯特菌的有效性。大肠杆菌K12被选为肠出血性食源性病原体大肠杆菌O157:H7的代表性微生物，无害李斯特菌分别作为常见食源性病原体单核细胞增生李斯特菌的替代微生物。用于消毒实验的液体基质是一种液体基质（MRD溶液）。本研究结果表明，高强度光脉冲处理比连续紫外线 - C或近紫外 - 可见光处理更快速、有效地灭活大肠杆菌和无害李斯特菌。在距离灯2.5厘米处使用高强度光脉冲，处理5秒后，大肠杆菌和无害李斯特菌的数量分别减少了3.07和3.77 log10 CFU/mL，在暴露于高强度光脉冲（106.2 J/cm²）30秒后，显示低于检测限（<0.22 log10 CFU/mL）。这些研究证明了替代非热光技术的杀菌效果及其作为食品工业中去污策略的潜力。
