Van Blerk M, Bossuyt X, Humbel R, Mewis A, Servais G, Tomasi J P, Van Campenhout C, Van Hoovels L, Vercammen M, Damoiseaux J, Coucke W, Van de Walle P
Acta Clin Belg. 2014 Apr;69(2):83-6. doi: 10.1179/2295333714Y.0000000010. Epub 2014 Mar 4.
Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97·7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.
针对核抗原的自身抗体，即抗核抗体（ANA）、双链DNA（dsDNA）抗体和可提取核抗原（ENA），可用作多种自身免疫性疾病的诊断标志物。2010年3月，比利时国家外部质量评估计划向所有进行ANA检测的临床实验室发送了一份由荷兰EASI（欧洲自身免疫标准化倡议）团队设计的关于ANA、抗dsDNA和抗ENA抗体检测的调查问卷。几乎所有实验室都完成了问卷（97.7%，127/130）。本文讨论了该问卷的结果，并提供了比利时实验室进行ANA、抗dsDNA和抗ENA抗体检测的最新技术的宝贵信息。此外，这项工作还提出了该计划咨询委员会成员根据这项研究结果制定的实用建议。
