甲硫氨酸脑啡肽治疗对大鼠视上核中加压素免疫反应性的性别特异性影响。

Sex-specific effects of met-enkephalin treatment on vasopressin immunoreactivity in the rat supraoptic nucleus.

作者信息

Blanco E, Carretero J, Sànchez F, Riesco J M, Vàzquez R

机构信息

Departmento de Ciencias Morfològicas, Biologìa Celular y Anatomìa Patològica (Anatomìa Humana). Facultad de Medicina, Universidad de Salamanca, Spain.

出版信息

Neuropeptides. 1989 Feb-Mar;13(2):115-20. doi: 10.1016/0143-4179(89)90008-5.

DOI:10.1016/0143-4179(89)90008-5

PMID:2472572

Abstract

The supraoptic nucleus of male and female rats treated with met-enkephalin or naloxone and met-enkephalin was examined with light microscopical immunocytochemistry for Arginine-vasopressin. Both genders exhibited the same distribution of immunostained magnocellular neurons. Met-enkephalin treatment caused an increase in number of immunostained vasopressin neurons. This effect was more pronounced in females than in males. Naloxone treatment diminished immunoreactive cytoplasmic vasopressin in males more effectively than in females. In enkephalin-treated animals numerous vasopressin immunoreactive varicosities appeared within the supraoptic nucleus, but were mostly absent in naloxone-treated animals and in controls. Our results indicate that met-enkephalin treatment either stimulates vasopressin synthesis or inhibits secretion. It is likely that steroid hormones mediate the action of enkephalin on vasopressin secretion in a specific manner.

摘要

采用光镜免疫细胞化学方法，对用甲硫氨酸脑啡肽或纳洛酮与甲硫氨酸脑啡肽处理的雄性和雌性大鼠的视上核进行精氨酸加压素检测。两种性别均表现出免疫染色的大细胞神经元分布相同。甲硫氨酸脑啡肽处理导致免疫染色的加压素神经元数量增加。这种效应在雌性中比在雄性中更明显。纳洛酮处理对雄性免疫反应性细胞质加压素的减少作用比对雌性更有效。在脑啡肽处理的动物中，视上核内出现了许多加压素免疫反应性曲张体，但在纳洛酮处理的动物和对照组中大多不存在。我们的结果表明，甲硫氨酸脑啡肽处理要么刺激加压素合成，要么抑制分泌。类固醇激素很可能以特定方式介导脑啡肽对加压素分泌的作用。