Carretero J, Sanchez D, Sanchez F, Montero M, Blanco E, Gonzalez R, Vazquez R
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Acta Histochem. 1990;89(2):121-30. doi: 10.1016/S0065-1281(11)80347-8.
Morphological and morphometric changes were found in adenohypophyseal GH immunoreactive cells after intraventricular administration of Met-enkephalin to adult rats of both sexes. Morphologically, following Met-enkephalin treatment, the males showed cells stained intensely and homogeneously in their cytoplasm, while the females showed cells with a weaker degree of staining and a cytoplasm with a granular aspect; previous administration of naloxone prevented the changes observed in the males and intensified the reaction in the females. Morphometrically, in all the groups of animals studied, the males showed a greater cellular area than the females, accompanied by a decrease in the cytoplasmic area but without significant differences in the nuclear area. Met-enkephalin in both sexes produced an increase in cellular area, accompanied by a significant increase in the cytoplasmic area and, although less manifest, in the nuclear area. Neither kind of change appeared when naloxone was administered prior to Met-enkephalin treatment.
对成年雌雄大鼠脑室内注射甲硫氨酸脑啡肽后，发现腺垂体生长激素免疫反应细胞出现形态学和形态计量学变化。形态学上，甲硫氨酸脑啡肽处理后，雄性大鼠细胞胞质染色强烈且均匀，而雌性大鼠细胞染色较弱，胞质呈颗粒状；预先注射纳洛酮可阻止雄性大鼠观察到的变化，并增强雌性大鼠的反应。形态计量学上，在所有研究的动物组中，雄性大鼠的细胞面积大于雌性大鼠，同时胞质面积减少，但核面积无显著差异。甲硫氨酸脑啡肽使雌雄大鼠的细胞面积均增加，同时胞质面积显著增加，核面积虽增加但不太明显。在甲硫氨酸脑啡肽处理前注射纳洛酮，则不会出现上述任何一种变化。
