在 FHV 和 NoV 混合感染期间，诺达病毒外壳蛋白和 RNA 进行差异分离，进入子代病毒体。
Differential segregation of nodaviral coat protein and RNA into progeny virions during mixed infection with FHV and NoV.
机构信息
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
出版信息
Virology. 2014 Apr;454-455:280-90. doi: 10.1016/j.virol.2014.03.003. Epub 2014 Mar 21.
Nodaviruses are icosahedral viruses with a bipartite, positive-sense RNA genome. The two RNAs are packaged into a single virion by a poorly understood mechanism. We chose two distantly related nodaviruses, Flock House virus and Nodamura virus, to explore formation of viral reassortants as a means to further understand genome recognition and encapsidation. In mixed infections, the viruses were incompatible at the level of RNA replication and their coat proteins segregated into separate populations of progeny particles. RNA packaging, on the other hand, was indiscriminate as all four viral RNAs were detectable in each progeny population. Consistent with the trans-encapsidation phenotype, fluorescence in situ hybridization of viral RNA revealed that the genomes of the two viruses co-localized throughout the cytoplasm. Our results imply that nodaviral RNAs lack rigorously defined packaging signals and that co-encapsidation of the viral RNAs does not require a pair of cognate RNA1 and RNA2.
小核糖核酸病毒是具有二十面体的病毒，其基因组为二分体、正链 RNA。这两种 RNA 通过一种尚未被充分了解的机制被包装到同一个病毒粒子中。我们选择了两种亲缘关系较远的小核糖核酸病毒，即 FHV 和 NHV，以探索病毒重组体的形成，以此进一步了解基因组识别和包装。在混合感染中，病毒在 RNA 复制水平上不兼容，它们的外壳蛋白分离成不同的子代颗粒群体。另一方面，RNA 包装是无差别地进行的，因为在每个子代群体中都可以检测到四种病毒 RNA。与跨包装表型一致，病毒 RNA 的荧光原位杂交显示，两种病毒的基因组在细胞质中完全共定位。我们的结果表明，小核糖核酸病毒的 RNA 缺乏严格定义的包装信号，并且病毒 RNA 的共包装不需要一对互补的 RNA1 和 RNA2。
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