一种新型的体外共培养系统允许同时分析成熟的生物膜和浮游细菌与人体肺上皮细胞。

A novel in vitro co-culture system allows the concurrent analysis of mature biofilm and planktonic bacteria with human lung epithelia.

机构信息

University of Manitoba, Winnipeg, Manitoba, Canada; Cangene Corporation, Winnipeg, Manitoba, Canada.

University of Manitoba, Winnipeg, Manitoba, Canada; National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Canada.

出版信息

J Microbiol Methods. 2014 Jun;101:49-55. doi: 10.1016/j.mimet.2014.03.018. Epub 2014 Apr 12.

DOI:10.1016/j.mimet.2014.03.018

PMID:24726869

Abstract

Pseudomonas aeruginosa establishes chronic infections by forming biofilms; however studies of the virulence have focused on the planktonic form. Few in vitro co-culture models exist to study biofilm infections. We present a novel in vitro co-culture method examining the interactions between mature P. aeruginosa biofilms and human lung epithelial cells.

摘要

铜绿假单胞菌通过形成生物膜来建立慢性感染；然而，对毒力的研究主要集中在浮游形式上。目前很少有体外共培养模型用于研究生物膜感染。我们提出了一种新的体外共培养方法，用于研究成熟铜绿假单胞菌生物膜与人肺上皮细胞之间的相互作用。