University of Manitoba, Winnipeg, Manitoba, Canada; Cangene Corporation, Winnipeg, Manitoba, Canada.
University of Manitoba, Winnipeg, Manitoba, Canada; National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Canada.
J Microbiol Methods. 2014 Jun;101:49-55. doi: 10.1016/j.mimet.2014.03.018. Epub 2014 Apr 12.
Pseudomonas aeruginosa establishes chronic infections by forming biofilms; however studies of the virulence have focused on the planktonic form. Few in vitro co-culture models exist to study biofilm infections. We present a novel in vitro co-culture method examining the interactions between mature P. aeruginosa biofilms and human lung epithelial cells.
铜绿假单胞菌通过形成生物膜来建立慢性感染；然而，对毒力的研究主要集中在浮游形式上。目前很少有体外共培养模型用于研究生物膜感染。我们提出了一种新的体外共培养方法，用于研究成熟铜绿假单胞菌生物膜与人肺上皮细胞之间的相互作用。
