Wagner Andreas Otto, Malin Cornelia, Lins Philipp, Gstraunthaler Gudrun, Illmer Paul
University of Innsbruck, Institute of Microbiology, Technikerstr. 25, A-6020 Innsbruck, Austria.
University of Innsbruck, Institute of Microbiology, Technikerstr. 25, A-6020 Innsbruck, Austria.
Anaerobe. 2014 Oct;29:29-33. doi: 10.1016/j.anaerobe.2014.03.012. Epub 2014 Apr 13.
A 750 m(3) anaerobic digester was studied over a half year period including a shift from good reactor performance to a reduced one. Various abiotic parameters like volatile fatty acids (VFA) (formic-, acetic-, propionic-, (iso-)butyric-, (iso-)valeric-, lactic acid), total C, total N, NH4 -N, and total proteins, as well as the organic matter content and dry mass were determined. In addition several process parameters such as temperature, pH, retention time and input of substrate and the concentrations of CH4, H2, CO2 and H2S within the reactor were monitored continuously. The present study aimed at the investigation of the abundance of acetogens and total cell numbers and the microbial methanogenic community as derived from PCR-dHPLC analysis in order to put it into context with the determined abiotic parameters. An influence of substrate quantity on the efficiency of the anaerobic digestion process was found as well as a shift from a hydrogenotrophic in times of good reactor performance towards an acetoclastic dominated methanogenic community in times of reduced reactor performance. After the change in substrate conditions it took the methano-archaeal community about 5-6 weeks to be affected but then changes occurred quickly.
对一个750立方米的厌氧消化器进行了为期半年的研究，其中包括从良好的反应器性能转变为性能下降的阶段。测定了各种非生物参数，如挥发性脂肪酸（VFA）（甲酸、乙酸、丙酸、（异）丁酸、（异）戊酸、乳酸）、总碳、总氮、铵态氮和总蛋白质，以及有机物含量和干物质。此外，还连续监测了几个工艺参数，如温度、pH值、停留时间和底物输入量，以及反应器内甲烷、氢气、二氧化碳和硫化氢的浓度。本研究旨在通过PCR-dHPLC分析研究产乙酸菌的丰度、总细胞数和微生物产甲烷群落，以便将其与所测定的非生物参数联系起来。研究发现底物量对厌氧消化过程的效率有影响，并且在反应器性能良好时从氢营养型转变为在反应器性能下降时以乙酸裂解为主导的产甲烷群落。底物条件改变后，甲烷古菌群落大约需要5至6周才受到影响，但随后变化迅速发生。
