花生铁调节转运蛋白1在烟草和水稻植株中的表达可改善铁营养状况。
Expression of peanut Iron Regulated Transporter 1 in tobacco and rice plants confers improved iron nutrition.
作者信息
Xiong Hongchun, Guo Xiaotong, Kobayashi Takanori, Kakei Yusuke, Nakanishi Hiromi, Nozoye Tomoko, Zhang Lixia, Shen Hongyun, Qiu Wei, Nishizawa Naoko K, Zuo Yuanmei
机构信息
Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
出版信息
Plant Physiol Biochem. 2014 Jul;80:83-9. doi: 10.1016/j.plaphy.2014.03.021. Epub 2014 Mar 31.
Iron (Fe) limitation is a widespread agricultural problem in calcareous soils and severely limits crop production. Iron Regulated Transporter 1 (IRT1) is a key component for Fe uptake from the soil in dicot plants. In this study, the peanut (Arachis hypogaea L.) AhIRT1 was introduced into tobacco and rice plants using an Fe-deficiency-inducible artificial promoter. Induced expression of AhIRT1 in tobacco plants resulted in accumulation of Fe in young leaves under Fe deficient conditions. Even under Fe-excess conditions, the Fe concentration was also markedly enhanced, suggesting that the Fe status did not affect the uptake and translocation of Fe by AhIRT1 in the transgenic plants. Most importantly, the transgenic tobacco plants showed improved tolerance to Fe limitation in culture in two types of calcareous soils. Additionally, the induced expression of AhIRT1 in rice plants also resulted in high tolerance to low Fe availability in calcareous soils.
铁（Fe）限制是石灰性土壤中普遍存在的农业问题，严重限制了作物产量。铁调节转运蛋白1（IRT1）是双子叶植物从土壤中吸收铁的关键成分。在本研究中，利用缺铁诱导型人工启动子将花生（Arachis hypogaea L.）AhIRT1导入烟草和水稻植株。在缺铁条件下，AhIRT1在烟草植株中的诱导表达导致幼叶中铁的积累。即使在铁过量条件下，铁浓度也显著提高，这表明铁状态并不影响转基因植株中AhIRT1对铁的吸收和转运。最重要的是，转基因烟草植株在两种石灰性土壤培养中对铁限制表现出更高的耐受性。此外，AhIRT1在水稻植株中的诱导表达也使其对石灰性土壤中低铁有效性具有高耐受性。
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