Zubkov V A, Gorshkova R P, Burtseva T I, Isakov V V, Ovodov Iu S
Bioorg Khim. 1989 Feb;15(2):187-91.
O-Specific polysaccharide has been isolated on mild acid hydrolysis of the lipopolysaccharide from Yersinia enterocolitica O: 4.32 (strain 96) and shown to consist of yersiniose B (3,6-dideoxy-4-C-(1-hydroxyethyl)-D-xylo-hexose, YerB) acetylated at C1' and 2-acetamido-2-deoxy-D-galactose residues in a molar ratio 1:2. Acid hydrolysis, methylation and 13C NMR studies indicated the polysaccharide to be composed of trisaccharide repeating units of the following structure: (sequence; see text) The data obtained revealed structural and serological interrelation between O-antigens of Y. enterocolitica O:4.32 and Y. intermedia O:4.33.
通过对小肠结肠炎耶尔森菌O:4.32(菌株96)脂多糖进行温和酸水解,分离得到了O-特异性多糖,结果表明其由在C1'位乙酰化的耶尔森糖B(3,6-二脱氧-4-C-(1-羟乙基)-D-木糖己糖,YerB)和2-乙酰氨基-2-脱氧-D-半乳糖残基组成,摩尔比为1:2。酸水解、甲基化和13C核磁共振研究表明该多糖由以下结构的三糖重复单元组成:(序列;见原文)所得数据揭示了小肠结肠炎耶尔森菌O:4.32和中间耶尔森菌O:4.33的O抗原之间的结构和血清学相互关系。