Li Jiuming, Yu Yuan, Qian Jun, Wang Yu, Zhang Jinghua, Zhi Jinfang
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Analyst. 2014 Jun 7;139(11):2806-12. doi: 10.1039/c4an00243a.
A novel integrated biosensor for biotoxicity assay has been developed by co-immobilizing microorganisms and mediators within a novel redox hydrogel. The proposed redox hydrogel acts as an immobilizing matrix both for microorganism E. coli and redox mediator, which was prepared by grafting the benzoquinone (BQ) redox mediator with gelatin/silica hybrid hydrogel (GSH). This redox hydrogel was characterized by UV-Vis, CV and EIS. The feasibility of the novel integrated biosensor for biotoxicity assay was demonstrated by measuring the heavy metal ions Hg(2+), Cu(2+) and Cd(2+) polluted water as the model toxicants. The results showed that the integrated biosensor was able to evaluate the water biotoxicity and the corresponding 50% inhibiting concentrations (IC50) are determined to be 21.2 μg mL(-1), 44 μg mL(-1) and 79 μg mL(-1), respectively. This integrated biosensor could achieve real-time monitoring of water quality and evaluation of biotoxicity. Moreover it avoids the waste and contamination of mediators, and also simplifies the assay process.
通过将微生物和介体共固定在新型氧化还原水凝胶中，开发了一种用于生物毒性测定的新型集成生物传感器。所提出的氧化还原水凝胶作为微生物大肠杆菌和氧化还原介体的固定基质，它是通过将苯醌（BQ）氧化还原介体与明胶/二氧化硅混合水凝胶（GSH）接枝制备而成。该氧化还原水凝胶通过紫外可见光谱、循环伏安法和电化学阻抗谱进行表征。以重金属离子Hg(2+)、Cu(2+)和Cd(2+)污染的水作为模型毒物，证明了这种新型集成生物传感器用于生物毒性测定的可行性。结果表明，该集成生物传感器能够评估水的生物毒性，相应的50%抑制浓度（IC50）分别为21.2 μg mL(-1)、44 μg mL(-1)和79 μg mL(-1)。这种集成生物传感器可以实现水质的实时监测和生物毒性评估。此外，它避免了介体的浪费和污染，还简化了测定过程。
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