Streicher R, Stoffel W
Institut für Biochemie, Medizinische Fakultät, Universität zu Köln.
Biol Chem Hoppe Seyler. 1989 May;370(5):503-10. doi: 10.1515/bchm3.1989.370.1.503.
The organization of the gene of the human myelin basic protein has been established. The gene contains seven exons distributed over 32-34 kb. The nucleotide sequences of the seven exons, extensive parts at the 5' noncoding region and upstream and downstream regions adjacent to the exons were sequenced. A comparison of the nucleotide sequence of the human gene with mouse MBP cDNA and parts of the gene structure of mouse MBP has been carried out. There is a high degree of conservation in the seven exons and the 5'-regulatory and part of the 3'-noncoding region: the nucleotide sequence of the coding region of the human and mouse gene show a 93% homology. As far as the reported 5'-noncoding and upstream regulatory regions of 478 bp are comparable these regions are more than 80% homologous. Primer extension experiments positioned the transcription initiation sites at -55, -82 and -183. In the 5'-regulatory region of MBP gene three direct repeats were found, a nonameric at -401/-416 and two octameric at -192/-216 and -492/-508. A decameric sequence (-256 to -265 from the translation start) is completely homologous to a sequence of the human PLP regulatory 5'-noncoding region.
人类髓鞘碱性蛋白基因的结构已被确定。该基因包含7个外显子，分布在32 - 34kb的区域内。对这7个外显子的核苷酸序列、5'非编码区的大片段以及与外显子相邻的上下游区域进行了测序。已将人类基因的核苷酸序列与小鼠MBP cDNA以及小鼠MBP基因结构的部分内容进行了比较。在7个外显子以及5'调控区和3'非编码区的一部分中存在高度保守性：人类和小鼠基因编码区的核苷酸序列显示出93%的同源性。就所报道的478bp的5'非编码区和上游调控区而言，这些区域的同源性超过80%。引物延伸实验将转录起始位点定位在-55、-82和-183处。在MBP基因的5'调控区发现了三个直接重复序列，一个位于-401/-416的九聚体，以及两个分别位于-192/-216和-492/-508的八聚体。一个十聚体序列（从翻译起始点起-256至-265）与人类PLP调控性5'非编码区的一个序列完全同源。
