Klymkowsky M W, Maynell L A
Molecular, Cellular, & Developmental Biology, University of Colorado, Boulder 80309-0347.
Dev Biol. 1989 Aug;134(2):479-85. doi: 10.1016/0012-1606(89)90121-8.
In Xenopus, one of the most dramatic events during oocyte maturation is the breakdown of the oocyte's asymmetrically organized system of cytokeratin-type intermediate filaments. Following oocyte maturation in vitro, we found that (1) the breakdown of cytokeratin filament organization proceeds in an animal to vegetal direction, (2) cytokeratin filament breakdown occurs normally in enucleated oocytes and so is independent of nuclear components, and (3) the injection of maturation-promoting factor (MPF) induces the breakdown of cytokeratin filaments. While the MPF-induced breakdown of the nuclear envelope is independent of new protein synthesis, the MPF-induced breakdown of cytokeratin filament organization requires the translation of maternal mRNAs. These results strongly suggest that the factors regulating cytokeratin reorganization in the oocyte are distinct from those involved in the breakdown of the nuclear envelope.
在非洲爪蟾中，卵母细胞成熟过程中最显著的事件之一是细胞角蛋白型中间丝不对称组织系统的解体。在体外卵母细胞成熟后，我们发现：（1）细胞角蛋白丝组织的解体从动物极向植物极进行；（2）细胞角蛋白丝的解体在去核卵母细胞中正常发生，因此与核成分无关；（3）注射成熟促进因子（MPF）会诱导细胞角蛋白丝的解体。虽然MPF诱导的核膜解体与新蛋白质合成无关，但MPF诱导的细胞角蛋白丝组织解体需要母源mRNA的翻译。这些结果有力地表明，调节卵母细胞中细胞角蛋白重组的因子与参与核膜解体的因子不同。
