Neste Oil Corporation, Technology Center Kilpilahti, P.O. Box 310, 06101 Porvoo, Finland.
Department of Chemistry, University of Helsinki, A.I.Virtasen aukio 1, P.O. Box 55, 00014 Helsinki, Finland.
J Anal Methods Chem. 2014;2014:498168. doi: 10.1155/2014/498168. Epub 2014 Mar 9.
The research was performed to study the simultaneous detection of a homologous series of α , ω -dicarboxylic acids (C2-C10), oxalic, malonic, succinic, glutaric, adipic, pimelic, suberic, azelaic, and sebacic acids, with capillary electrophoresis using indirect UV detection. Good separation efficiency in 2,6-pyridinedicarboxylic acid as background electrolyte modified with myristyl trimethyl ammonium bromide was obtained. The dicarboxylic acids were ionised and separated within five minutes. For the study, authentic samples were collected onto dry cellulose membrane filters of a cascade impactor (12 stages) from outdoor spring aerosols in an urban area. Hot water and ultrasonication extraction methods were used to isolate the acids from membrane filters. Due to the low concentrations of acids in the aerosols, the extracts were concentrated with solid-phase extraction (SPE) before determination. The enrichment of the carboxylic acids was between 86 and 134% with sample pretreatment followed by 100-time increase by preparation of the sample to 50 μ L. Inaccuracy was optimised for all the sample processing steps. The aerosols contained dicarboxylic acids C2-C10. Then, mostly they contained C2, C5, and C10. Only one sample contained succinic acid. In the study, the concentrations of the acids in aerosols were lower than 10 ng/m(3).
本研究旨在采用毛细管电泳-间接紫外检测法同时检测α,ω-二羧酸（C2-C10）、草酸、丙二酸、琥珀酸、戊二酸、己二酸、庚二酸、辛二酸、壬二酸和癸二酸。在以 2,6-吡啶二甲酸为背景电解质，并用十四烷基三甲基溴化铵修饰的条件下，可获得良好的分离效率。这些二羧酸在五分钟内即可实现离子化和分离。在这项研究中，从城市地区户外春季气溶胶中使用级联冲击器（12 级）的干燥纤维素膜过滤器收集到真实的样本。采用热水和超声提取法从膜过滤器中分离出酸。由于气溶胶中酸的浓度较低，在测定前采用固相萃取（SPE）浓缩提取液。羧酸的富集率在 86%至 134%之间，通过预处理样品并将样品制备至 50 μL，可使样品增加 100 倍。优化了所有样品处理步骤的准确度。气溶胶中含有二羧酸 C2-C10。然后，它们主要含有 C2、C5 和 C10。只有一个样本中含有琥珀酸。在研究中，气溶胶中酸的浓度低于 10 ng/m(3)。
