Pol J Microbiol. 2013;62(4):365-72.
A disk-diffusion method experiment assessed the impact of nanosilver on production of secondary metabolites (pigments) by the Fusarium culmorum fungus. Nanosilver colloidal particles in water have been obtained by the use of a method based on high voltage electric arcs between silver electrodes. The silver nanoparticles size in colloid ranged between 15 and 100 nm and 7, 35 and 70 ppm concentration. Nanosilver modifies the metabolism of the researched F. culmorum strain. Coming into contact with nanosilver colloids induces more intensive mycelia pigmentation correlated with nanosilver concentration levels. The performed analysis of metabolites indicates that under the influence of nanosilver fungi biosynthesise aurofusarin more intensively and the conversion of rubrofusarin to aurofusarin is intensified as compared to the control culture. Under the influence of nanosilver F. culmorum intensively biosynthesises an unidentified dye which shares structural features with aurofusarin but which is not produced by fungi in standard cultures.
一种圆盘扩散方法实验评估了纳米银对尖孢镰刀菌产生次生代谢物（色素）的影响。通过使用基于银电极之间高压电弧的方法，在水中获得了纳米银胶体颗粒。胶体中的银纳米颗粒尺寸在 15 至 100nm 之间，浓度为 7、35 和 70ppm。纳米银改变了研究的尖孢镰刀菌菌株的代谢。与纳米银胶体接触会引起更强烈的菌丝体着色，这与纳米银浓度水平相关。对代谢物的分析表明，在纳米银的影响下，真菌更强烈地生物合成金晕菌素，并且与对照培养物相比，金晕菌素向rubrofusarin 的转化率增强。在纳米银的影响下，尖孢镰刀菌强烈地生物合成一种未知的染料，该染料与金晕菌素具有结构特征，但在标准培养物中真菌不产生。
