Pol J Microbiol. 2013;62(4):439-43.
The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.
水质卫生状况的诊断评估主要基于对保留在膜滤器上的细菌的培养。然而，经典的微生物学方法有很多缺点。越来越多的情况下，对水中存在的病原体的快速检测和鉴定是基于分子生物学技术。本研究的目的是确定实时 PCR 方法与推荐的细菌培养方法相比，在水样中病原体诊断方面的有效性和实用性。该研究涉及检测和鉴定水的主要卫生指标，如：沙门氏菌、大肠杆菌、金黄色葡萄球菌和产气荚膜梭菌。分析采用了受污染的参考材料（上述细菌）和真实环境样本的水样本进行，使用实时 PCR 方法检测和鉴定水样中沙门氏菌、大肠杆菌、金黄色葡萄球菌和产气荚膜梭菌的核酸。
