Dutt K, York D, Kaplan H J, Semple E, Verly G, Srinivasan A
Department of Pathology, Morehouse School of Medicine, Atlanta, Georgia 30310.
Invest Ophthalmol Vis Sci. 1989 Jul;30(7):1535-41.
The ability of the human immunodeficiency virus type 1 (HIV-1) to replicate in cells derived from ocular tissue was studied. Primary retinal cultures (containing both glial and neuronal cells) were found to support the replication of HIV upon transfection with molecularly cloned proviral DNA. In addition, established retinal pigment epithelial (RPE) cell lines also produced HIV particles upon transfection. HIV released by these cell lines was able to infect and induce characteristic cytopathic effects in T4+ cells. An indicator plasmid containing the HIV long terminal repeat sequences (LTR) linked to the chloramphenicol acetyltransferase gene showed barely detectable activity in RPE cells and was transactivated by the addition of the HIV "tat" gene. Based on these observations, direct infection of ocular tissue derived cells such as RPE, fetal retinal cells, retinoblastoma cells (Y 79, WER1), choroidal endothelial cells (Chor 55) (mix culture) and corneal fibroblasts (K61) by HIV was attempted. HIV replication in these cells was not detected by reverse transcriptase, antigen and transactivation function assays.
研究了1型人类免疫缺陷病毒（HIV-1）在源自眼部组织的细胞中复制的能力。发现原代视网膜培养物（包含神经胶质细胞和神经元细胞）在用分子克隆的前病毒DNA转染后能够支持HIV的复制。此外，已建立的视网膜色素上皮（RPE）细胞系在转染后也产生HIV颗粒。这些细胞系释放的HIV能够感染T4+细胞并诱导特征性细胞病变效应。含有与氯霉素乙酰转移酶基因相连的HIV长末端重复序列（LTR）的指示质粒在RPE细胞中显示出几乎检测不到的活性，并通过添加HIV“tat”基因而被反式激活。基于这些观察结果，尝试了HIV对源自眼部组织的细胞如RPE、胎儿视网膜细胞、视网膜母细胞瘤细胞（Y79、WER1）、脉络膜内皮细胞（Chor55）（混合培养）和角膜成纤维细胞（K61）的直接感染。通过逆转录酶、抗原和反式激活功能测定未检测到这些细胞中的HIV复制。
