Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong.
Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong.
Food Chem. 2014 Sep 1;158:262-9. doi: 10.1016/j.foodchem.2014.02.119. Epub 2014 Mar 1.
This study examined the potential of two-step fermentation to preserve TPs in functional soy-tea beverage. Fermented soymilk-tea (FST) was produced by culturing Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus and Bifidobacterium longum in soymilk supplemented with tea extract (TE). Total phenolic content (TPC) and anti-radical activities were determined for FSTs and fermented soymilk (FS). A HPLC method was employed to quantify nine major tea phenolics in FST products. TPC was significantly higher (p<0.05) in FST than FS, in the order of green tea>oolong tea>black tea>soymilk. The FSTs were effective at scavenging DPPH-radical rather than hydroxyl radical. Optimal pH to stabilize TPs in SMT was ca. 5.7, which reduced total TP loss by ca. 40% compared with that obtained from products with TE supplemented at the beginning of fermentation. A gradual decrease in TPs was observed during storage (4 °C), with more than half of total TPs remained in FST after 8 weeks.
本研究探讨了两步发酵法在功能性豆茶饮料中保存茶多酚(TPs)的潜力。通过在添加茶提取物(TE)的豆浆中培养嗜热链球菌、德氏乳杆菌保加利亚亚种和长双歧杆菌,生产发酵豆茶(FST)。测定 FST 和发酵豆浆(FS)的总酚含量(TPC)和自由基清除活性。采用 HPLC 法测定 FST 产品中 9 种主要茶酚的含量。FST 的 TPC 明显高于 FS,顺序为绿茶>乌龙茶>红茶>豆浆。FST 对 DPPH 自由基的清除效果优于羟自由基。在 SMT 中稳定 TPs 的最佳 pH 值约为 5.7,与发酵开始时添加 TE 的产品相比,总 TP 损失减少约 40%。在储存(4°C)过程中观察到 TPs 逐渐减少,8 周后 FST 中仍保留超过一半的总 TPs。