Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná, Rua Cel. Francisco H. dos Santos, 100, CEP 81531-990 Curitiba, Paraná, Brazil.
Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná, Rua Cel. Francisco H. dos Santos, 100, CEP 81531-990 Curitiba, Paraná, Brazil.
Vet Parasitol. 2014 May 28;202(3-4):128-31. doi: 10.1016/j.vetpar.2014.03.022. Epub 2014 Mar 30.
Sera from 112 mares from 5 horse-breeding farms was examined for the presence of antibodies to Neospora caninum and Toxoplasma gondii by an indirect fluorescent antibody test (IFAT), as well as from dogs and cattle present on these properties for the presence of antibodies to N. caninum. Among the 112 mares, 35 had a history of reproductive problems in the last breeding season and 77 had no reproductive problems. The rates of seroprevalence of N. caninum in mares with and without a history of reproductive problems were 25.71% and 6.49% and from T. gondii 2.85% and 1.29%, respectively. In dogs and cattle, the rates of seroprevalence of N. caninum were 10.52% and 15.55%, respectively. A positive correlation was found between the presence of antibodies against N. caninum (p=0.010) in mares and the occurrence of reproductive problems using the Fisher's exact test. Significantly higher seroprevalence for N. caninum in mares was observed on the farm that had seropositive dogs (p=0.018). Cattle on this farm were also seropositive. No significant differences in seropositivity were found on farms where dogs were seronegative, or absent. This result suggests, for the first time, the presence of seropositive dogs as a risk factor for N. caninum in mares and the necessity for further investigation of the epidemiology of this parasite in horse-breeding farms with reproductive problems and the presence of cattle and dogs. This is the first report on the occurrence of antibodies against N. caninum in horses from the state of Santa Catarina, Brazil.
从 5 个马场的 112 匹母马的血清中，通过间接荧光抗体试验（IFAT）检测了抗 Neospora caninum 和 Toxoplasma gondii 的抗体，并检测了这些马场中的狗和牛是否存在抗 Neospora caninum 的抗体。在 112 匹母马中，有 35 匹在最近的繁殖季节有繁殖问题史，77 匹没有繁殖问题史。有繁殖问题史和无繁殖问题史的母马的 Neospora caninum 血清阳性率分别为 25.71%和 6.49%，Toxoplasma gondii 的血清阳性率分别为 2.85%和 1.29%。在狗和牛中，Neospora caninum 的血清阳性率分别为 10.52%和 15.55%。使用 Fisher 精确检验发现，母马血清中抗 Neospora caninum 的抗体存在与繁殖问题的发生之间存在正相关关系（p=0.010）。在有血清阳性犬的农场中，母马的 Neospora caninum 血清阳性率显著更高（p=0.018）。该农场的牛也呈血清阳性。在没有血清阳性犬的农场中，母马的血清阳性率没有显著差异，也没有犬存在。这是首次表明血清阳性犬作为马繁殖问题和存在牛和犬的马场中 Neospora caninum 的风险因素的存在，并且需要进一步调查这些寄生虫的流行病学。这是巴西圣卡塔琳娜州首次报告马的抗 Neospora caninum 抗体的发生。
