Dreno B, Hallet M M, Moisan J P, Soulillou J P, Jacques Y
Service de Dermatologie, Centre Hospitalo-Universitaire, Nantes, France.
J Invest Dermatol. 1989 Jul;93(1):78-82. doi: 10.1111/1523-1747.ep12277358.
The antirecombinant interleukin 2 (rec-IL-2) monoclonal antibody (moAb) 15.2 cross-reacts with a skin antigen located at the cell surface of human keratinocytes within the granular layer. This study extends the analysis of this IL-2-like material to its reactivity with eight antibodies raised against natural IL-2, rec-IL-2 or IL-2 peptides. Four among them were found to react with the granular epidermal layer as well as with a simian virus 40 (SV40) transformed human keratinocyte cell line. Each of these four antibodies gave similar labeling patterns, although with different intensities, and competitively inhibited each other. Analysis at the messenger RNA level in epidermal cells and SVK 14 also indicated that this material is very likely different from IL-2. From the knowledge, for some of these antibodies, of the amino-acid regions they recognize on the IL-2 molecule, it is inferred that the skin antigen shares with IL-2 at least two overlapping epitopes located in the 33-54 amino-acid region of IL-2, a region that has been shown to be involved in the binding of IL-2 to the IL-2-receptor (IL-2-R) 55 kD chain. Indeed, a purified recombinant soluble species of this IL-2-R is shown in this study to bind specifically to the IL-2-like skin material. As far as IL-2-R bearing cells are found in normal epidermis (Langerhans cells) and as important infiltrates of IL-2-R positive T lymphocytes are often encountered in cutaneous diseases, a potential role for this IL-2-like material in skin immunophysiopathology is suggested.
抗重组白细胞介素2（rec-IL-2）单克隆抗体（moAb）15.2与位于颗粒层人角质形成细胞表面的一种皮肤抗原发生交叉反应。本研究将对这种白细胞介素2样物质的分析扩展至其与8种针对天然IL-2、rec-IL-2或IL-2肽产生的抗体的反应性。其中4种抗体被发现可与颗粒状表皮层以及猿猴病毒40（SV40）转化的人角质形成细胞系发生反应。这4种抗体中的每一种都给出了相似的标记模式，尽管强度不同，并且相互之间具有竞争性抑制作用。对表皮细胞和SVK 14中信使RNA水平的分析也表明，这种物质很可能与IL-2不同。根据其中一些抗体所识别的IL-2分子上的氨基酸区域的知识，可以推断皮肤抗原与IL-2至少共享位于IL-2的33-54氨基酸区域的两个重叠表位，该区域已被证明参与IL-2与IL-2受体（IL-2-R）55 kD链的结合。实际上，本研究表明这种IL-2-R的纯化重组可溶性形式可特异性结合IL-2样皮肤物质。鉴于在正常表皮中发现有携带IL-2-R的细胞（朗格汉斯细胞），并且在皮肤疾病中经常会遇到大量IL-2-R阳性T淋巴细胞浸润，提示这种IL-2样物质在皮肤免疫生理病理学中可能具有作用。
