Chen Yu-Yue, Liu Xin-Wei, Gong Tian-Xing, Zhang Zhi-Yu, Liu Yun-En, Zhang Yu-Biao, Xiang Liang-Bi, Xia Hong
Department of Orthopedics, General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 5000, Guangdong Province, China.
Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110840, Liaoning Province, China.
Clin Neurol Neurosurg. 2014 May;120:1-5. doi: 10.1016/j.clineuro.2014.02.004. Epub 2014 Feb 18.
Amyotrophic lateral sclerosis (ALS) or motor neuron disease is an adult-onset progressive neurodegenerative disorder. ALS-CSF has been shown to produce toxic effects on motor neuron cells like aberrant neurofilament phosphorylation and morphological alterations of nuclear and soma size. Our current study was designed to investigate the neuroprotective role of platelet derived growth factor (PDGF) in reverting the adverse effects produced by ALS-CSF.
Our present study was carried out to determine the restorative potential of PDGF on the toxic effects of ALS-CSF on NSC motor neuron cells from patients. Therefore the cells were divided in to three groups: (a) normal control (NC) - the cells were not exposed to ALS-CSF; (b) ALS group - the cells were exposed to ALS-CSF; (c) NALS group - the cells were exposed to non ALS CSF. Further each of these groups was supplemented with PDGF.
We observed that the mean area of nucleus and cell soma of the differentiated NSC motor neuron cells was significantly reduced in the cells exposed to ALS-CSF. We also observed that subsequent treatment with PDGF restored the soma area and nucleus of the ALS-CSF exposed cells significantly. Taken together, we show that supplementation with PDGF restores the morphological changes induced by ALS-CSF and PDGF may play a significant role in protecting motor neurons from apoptosis in ALS and thereby it promoting the cell survival.
肌萎缩侧索硬化症（ALS）或运动神经元病是一种成人起病的进行性神经退行性疾病。ALS患者的脑脊液（ALS-CSF）已被证明对运动神经元细胞产生毒性作用，如异常的神经丝磷酸化以及细胞核和细胞体大小的形态改变。我们当前的研究旨在探讨血小板衍生生长因子（PDGF）在逆转ALS-CSF产生的不利影响方面的神经保护作用。
我们目前的研究旨在确定PDGF对ALS-CSF对患者神经干细胞（NSC）运动神经元细胞毒性作用的恢复潜力。因此，将细胞分为三组：（a）正常对照组（NC）——细胞未暴露于ALS-CSF；（b）ALS组——细胞暴露于ALS-CSF；（c）非ALS组——细胞暴露于非ALS患者的脑脊液。此外，这些组中的每一组都补充了PDGF。
我们观察到，暴露于ALS-CSF的分化NSC运动神经元细胞的细胞核和细胞体平均面积显著减小。我们还观察到，随后用PDGF处理可显著恢复暴露于ALS-CSF的细胞的细胞体面积和细胞核。综上所述，我们表明补充PDGF可恢复由ALS-CSF诱导的形态变化，并且PDGF可能在保护ALS中的运动神经元免受凋亡从而促进细胞存活方面发挥重要作用。
