Ong L Y, Pang T, Lim S H, Tan E L, Puthucheary S D
Department of Genetics and Cellular Biology, University of Malaya, Kuala Lumpur.
J Med Microbiol. 1989 Jul;29(3):195-8. doi: 10.1099/00222615-29-3-195.
A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed.
本文描述了一种用于检测伤寒患者中IgM抗体的简易黏附试验。该试验采用IgM“捕获”法，即将待测血清加到预先包被有抗人IgM的微量滴定板孔中，随后加入经染色的伤寒沙门菌抗原悬液，阳性病例中会出现黏附现象。通过此试验，61例确诊伤寒病例的血清中有58例（95%）含有针对伤寒沙门菌H或O抗原或两者的IgM抗体。在仅依据肥达试验效价显著而诊断为伤寒的患者中，76例血清中有31例（41%）含有针对伤寒沙门菌H或O抗原或两者的IgM抗体。检测到IgM抗体存在一些交叉反应，尤其是与甲型副伤寒沙门菌和乙型副伤寒沙门菌的O抗原。82例非伤寒热（钩端螺旋体病、斑疹伤寒、登革热）患者的血清在该试验中无反应。正常血清中未检测到针对伤寒沙门菌O抗原的IgM，仅有少数（3.9%）对H抗原有低水平的IgM。本文讨论了这种简易、灵敏、特异且经济的试验的意义和潜在重要性。
